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dc.contributor.authorAssar, Shokrollahen_US
dc.contributor.authorArababadi, Mohammad Kazemien_US
dc.contributor.authorMohit, Maryamen_US
dc.contributor.authorAhmadabadi, Behzad Nasirien_US
dc.contributor.authorPumpens, Paulen_US
dc.contributor.authorKhorramdelazad, Hosseinen_US
dc.contributor.authorHajghani, Masomehen_US
dc.contributor.authorAssar, Sepidehen_US
dc.contributor.authorAraste, Majiden_US
dc.contributor.authorNekhei, Zohreen_US
dc.contributor.authorSendi, Hosseinen_US
dc.contributor.authorKennedy, Dereken_US
dc.date.accessioned2017-05-03T11:12:34Z
dc.date.available2017-05-03T11:12:34Z
dc.date.issued2012en_US
dc.date.modified2014-08-13T23:52:18Z
dc.identifier.issn14336510en_US
dc.identifier.urihttp://hdl.handle.net/10072/46900
dc.description.abstractBackground: Escape mutations potentially allow viruses to avoid detection and clearance by the host immune sys-tem and may represent a mechanism through which infections may persist in some patients. The association of the mutations in the HBcAg gene with Hepatitis B asymptomatic carriers (ASC) has not been studied adequately. The current study was aimed to investigate HBcAg18-27 CTL epitope mutations in ASC patients in the South-Eastern region of Iran. Methods: 100 ASC patients were selected for this study and screened for HLA-A2 using flow cytometry. HBV-DNA was extracted from the HLA-A2 positive patients and the HBc gene was amplified using PCR. Direct double sequencing was performed to analyse mutations in the HBc gene of HBV isolates from patients with ASC. Results: Overall, 25 (25%) of individuals were HLA-A2 positive. Direct double sequencing indicated no mutations in the HBcAg18-27 epitope. However, four mutations within the T helper and three mutations within the B cell epitopes of ASC patients were identified. Conclusions: The lack of mutations within the HBcAg18-27 epitope suggests that the antigenicity of this region is not altered in HBV isolates of our patients and therefore antigen presentation would occur normally to the pa-tient's immune system through HLA-A2. However, in the course of this study we revealed some novel mutations within the T helper and B cell epitopes that may affect the efficiencies of immune response of ASC patients against these novel HBV epitopes. (Clin. Lab. 2012;58:53-60)en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_US
dc.format.extent619904 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglishen_US
dc.language.isoen_US
dc.publisherClinical Laboratory Publicationsen_US
dc.publisher.placeGermanyen_US
dc.publisher.urihttp://www.clin-lab-publications.com/issue/79en_US
dc.relation.ispartofstudentpublicationNen_US
dc.relation.ispartofpagefrom53en_US
dc.relation.ispartofpageto60en_US
dc.relation.ispartofjournalClinical Laboratoryen_US
dc.relation.ispartofvolume58en_US
dc.rights.retentionYen_US
dc.subject.fieldofresearchGastroenterology and Hepatologyen_US
dc.subject.fieldofresearchMedical Genetics (excl. Cancer Genetics)en_US
dc.subject.fieldofresearchcode110307en_US
dc.subject.fieldofresearchcode110311en_US
dc.titleT Helper and B Cell Escape Mutations within the HBc Gene in Patients with Asymptomatic HBV Infection: A Study From the South-Eastern Region of Iranen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.rights.copyrightCopyright 2012 Clinical Laboratory Publications. The attached file is reproduced here in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.en_US
gro.date.issued2012
gro.hasfulltextFull Text


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