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dc.contributor.authorBoon, Connieen_US
dc.contributor.authorL. Hawkins, Clareen_US
dc.contributor.authorBisht, Kavitaen_US
dc.contributor.authorS. Coombes, Jeffen_US
dc.contributor.authorBakrania, Bhavishaen_US
dc.contributor.authorWagner, Karl-Heinzen_US
dc.contributor.authorBulmer, Andrewen_US
dc.date.accessioned2017-04-24T13:19:28Z
dc.date.available2017-04-24T13:19:28Z
dc.date.issued2012en_US
dc.date.modified2013-06-03T23:26:29Z
dc.identifier.issn08915849en_US
dc.identifier.doi10.1016/j.freeradbiomed.2012.03.002en_US
dc.identifier.urihttp://hdl.handle.net/10072/47257
dc.description.abstractA protective association between bilirubin and atherosclerosis/ischemic heart disease clearly exists in vivo. However, the relationship between bilirubin and in vivo oxidative stress parameters in a clinical population remains poorly described. The aim of this study was to assess whether persons expressing Gilbert syndrome (GS; i.e., unconjugated hyperbilirubinemia) are protected from thiol oxidation and to determine if this, in addition to their improved lipoprotein profile, could explain reduced oxidized low-density lipoprotein (oxLDL) status in them. Forty-four matched GS and control subjects were recruited and blood was prepared for the analysis of lipid profile and multiple plasma antioxidants and measures of oxidative stress. GS subjects possessed elevated plasma reduced thiol (8.03ᱮ09 versus 6.75ᱮ39 nmol/mg protein; P<0.01) and glutathione concentrations (12.7Ხ39 versus 9.44Ხ45 卻 P<0.001). Oxidative stress status (reduced:oxidized glutathione; GSH:GSSG) was significantly improved in GS (0.49ᰮ16 versus 0.32ᰮ12; P<0.001). Protein carbonyl concentrations were negatively associated with bilirubin concentrations and were significantly lower in persons with >40 占bilirubin versus controls (<17.1 孯l/L; P<0.05). Furthermore, absolute oxLDL concentrations were significantly lower in GS subjects (P<0.05). Forward stepwise regression analysis revealed that bilirubin was associated with increased GSH:GSSG ratio and reduced thiol concentrations, which, in addition to reduced circulating LDL, probably decreased oxLDL concentrations within the cohort. In addition, a marked reduction in total cholesterol concentrations in hyperbilirubinemic Gunn rats is presented (Gunn 0.57ᰮ09 versus control 1.69ᰮ40 mmol/L; P<0.001), arguing for a novel role for bilirubin in modulating lipid status in vivo. These findings implicate the physiological importance of bilirubin in protecting from atherosclerosis by reducing thiol and subsequent lipoprotein oxidation, in addition to reducing circulating LDL concentrationsen_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_US
dc.languageEnglishen_US
dc.language.isoen_US
dc.publisherElsevieren_US
dc.publisher.placeAustraliaen_US
dc.relation.ispartofstudentpublicationYen_US
dc.relation.ispartofpagefrom2120en_US
dc.relation.ispartofpageto2127en_US
dc.relation.ispartofissue10en_US
dc.relation.ispartofjournalFree Radical Biology and Medicineen_US
dc.relation.ispartofvolume52en_US
dc.rights.retentionYen_US
dc.subject.fieldofresearchCardiology (incl. Cardiovascular Diseases)en_US
dc.subject.fieldofresearchcode110201en_US
dc.titleReduced circulating oxidized LDL is associated with hypocholesterolemia and enhanced thiol status in Gilbert syndromeen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.facultyGriffith Health, School of Medical Scienceen_US
gro.date.issued2012
gro.hasfulltextNo Full Text


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