Show simple item record

dc.contributor.authorA. Guyton, Roberten_US
dc.contributor.authorE. Halkos, Michaelen_US
dc.contributor.authorHeadrick, Johnen_US
dc.contributor.authorJ. Zatta, Amandaen_US
dc.contributor.authorKerendi, Farazen_US
dc.contributor.authorKin, Hajimeen_US
dc.contributor.authorS. Amerson, Bradleyen_US
dc.contributor.authorT. Lofye, Marken_US
dc.contributor.authorVinten-Johansen, Jakoben_US
dc.contributor.authorZhao, Zhi-Qingen_US
dc.date.accessioned2017-04-04T14:28:53Z
dc.date.available2017-04-04T14:28:53Z
dc.date.issued2005en_US
dc.date.modified2009-09-23T02:38:08Z
dc.identifier.issn00086363en_US
dc.identifier.doi10.1016/j.cardiores.2005.02.015en_AU
dc.identifier.urihttp://hdl.handle.net/10072/4737
dc.description.abstractObjective This study tested the hypothesis that brief cycles of iterative ischemia-reperfusion at onset of reperfusion (termed "postconditioning", post-con) delays washout of intravascular adenosine and thereby increases endogenous adenosine receptor (AR) activation during the early moments of reperfusion (R). Methods Isolated mouse hearts were subjected to 20 min global ischemia (I) and 30 min R with or without post-con (3 or 6 cycles of 10 s R&I). Intravascular purines in coronary effluent were analyzed by HPLC. To assess the functional role of endogenous AR activation in post-con, an open-chest rat model of myocardial infarction was employed. Rats were randomly divided into 11 groups: control, no intervention at R; post-con, three cycles of 10 s R followed by 10 s LCA re-occlusion immediately upon R. In the following interventions, drugs (or vehicle) were administered 5 min before R in the absence or presence (᩠of post-con. Vehicle (DMSO <300 嬯kg); 8-SPT (non-selective AR antagonist, 10 mg/kg) ᠰost-con; DPCPX (A1AR antagonist, 0.1 mg/kg) ᠰost-con; ZM241385 (A2AAR antagonist, 0.2 mg/kg) ᠰost-con; MRS1523 (A3AR antagonist, 2 mg/kg) ᠰost-con. Results In isolated mouse hearts, post-con reduced diastolic pressure during both early (26 ᠳ* vs. 37 ᠳ mmHg at 5 min) and late (22 ᠳ* vs. 34 ᠳ mmHg at 30 min) R. Post-con also hastened the early recovery of contractile function (developed pressure 39 ᠶ* vs. 16 ᠲ mmHg at 5 min R), although differences did not persist at 30 min R. Importantly, post-con was associated with reduced adenosine washout (58 ᠵ* vs. 155 ᠱ6 nM/min/g) at 2 min R suggesting greater retention time of intravascular adenosine. In rats, post-con significantly attenuated infarct size compared to control (40 ᠳ% vs. 53 ᠲ%* in control), an effect that was unaltered by DPCPX (42 ᠲ%) but was abrogated by 8-SPT (50 ᠲ%), ZM241385 (49 ᠳ%) or MRS1523 (52 ᠱ%) (P<0.02). Conclusion These data suggest that post-con involves endogenous activation of A2A and A3 but not A1AR subtypes. This activation may be linked to the delay in the washout of intravascular adenosine during the early minutes of R during which post-con is applied.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_AU
dc.languageEnglishen_US
dc.language.isoen_AU
dc.publisherElsevier BVen_US
dc.publisher.placeNetherlandsen_US
dc.relation.ispartofstudentpublicationNen_AU
dc.relation.ispartofpagefrom124en_US
dc.relation.ispartofpageto133en_US
dc.relation.ispartofissue1en_AU
dc.relation.ispartofjournalCardiovascular Researchen_US
dc.relation.ispartofvolume67en_US
dc.rights.retentionNen_AU
dc.subject.fieldofresearchcode320603en_US
dc.titlePostconditioning reduces infarct size via adenosine receptor activation by endogenous adenosineen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.rights.copyrightCopyright 2005 Elsevier : Reproduced in accordance with the copyright policy of the publisher : This journal is available online - use hypertext links.en_AU
gro.date.issued2005
gro.hasfulltextNo Full Text


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record