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dc.contributor.authorSpeers-Roesch, B
dc.contributor.authorRichards, JG
dc.contributor.authorBrauner, CJ
dc.contributor.authorFarrell, AP
dc.contributor.authorHickey, AJR
dc.contributor.authorWang, YS
dc.contributor.authorRenshaw, GMC
dc.date.accessioned2017-11-29T12:00:28Z
dc.date.available2017-11-29T12:00:28Z
dc.date.issued2012
dc.date.modified2013-06-11T04:04:53Z
dc.identifier.issn0022-0949
dc.identifier.doi10.1242/jeb.059642
dc.identifier.urihttp://hdl.handle.net/10072/47573
dc.description.abstractThe critical O2 tension of whole-animal O2 consumption rate (Embedded Image), or Pcrit, is the water PO2 (PwO2) at which an animal transitions from an oxyregulator to an oxyconformer. Although Pcrit is a popular measure of hypoxia tolerance in fishes because it reflects the capacity for O2 uptake from the environment at low PwO2, little is known about the interrelationships between Pcrit and blood O2 transport characteristics and increased use of anaerobic metabolism during hypoxia exposure in fishes, especially elasmobranchs. We addressed this knowledge gap using progressive hypoxia exposures of two elasmobranch species with differing hypoxia tolerance. The Pcrit of the hypoxia-tolerant epaulette shark (Hemiscyllium ocellatum, 5.10±0.37 kPa) was significantly lower than that of the comparatively hypoxia-sensitive shovelnose ray (Aptychotrema rostrata, 7.23±0.40 kPa). Plasma [lactate] was elevated above normoxic values at around Pcrit in epaulette sharks, but increased relative to normoxic values at PwO2 below Pcrit in shovelnose rays, providing equivocal support for the hypothesis that Pcrit is associated with increased anaerobic metabolism. The Embedded Image, arterial PO2 and arterial blood O2 content (CaO2) were similar between the two species under normoxia and decreased in both species with progressive hypoxia, but as PwO2 declined, epaulette sharks had a consistently higher Embedded Image and CaO2 than shovelnose rays, probably due to their significantly greater in vivo haemoglobin (Hb)–O2 binding affinity (in vivo Hb–O2 P50=4.27±0.57 kPa for epaulette sharks vs 6.35±0.34 kPa for shovelnose rays). However, at PwO2 values representing the same percentage of each species' Pcrit (up to ∼175% of Pcrit), Hb–O2 saturation and CaO2 were similar between species. These data support the hypothesis that Hb–O2 P50 is an important determinant of Pcrit and suggest that Pcrit can predict Hb–O2 saturation and CaO2 during hypoxia exposure, with a lower Pcrit being associated with greater O2 supply at a given PwO2 and consequently better hypoxia tolerance. Thus, Pcrit is a valuable predictor of environmental hypoxia tolerance and hypoxia exposures standardized at a given percentage of Pcrit will yield comparable levels of arterial hypoxaemia, facilitating cross-species comparisons of responses to hypoxia.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherThe Company of Biologists
dc.publisher.placeUnited Kingdom
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom93
dc.relation.ispartofpageto102
dc.relation.ispartofissue1
dc.relation.ispartofjournalJournal of Experimental Biology
dc.relation.ispartofvolume215
dc.rights.retentionY
dc.subject.fieldofresearchBiological Sciences not elsewhere classified
dc.subject.fieldofresearchBiological Sciences
dc.subject.fieldofresearchMedical and Health Sciences
dc.subject.fieldofresearchcode069999
dc.subject.fieldofresearchcode06
dc.subject.fieldofresearchcode11
dc.titleHypoxia tolerance in elasmobranchs. I. Critical oxygen tension as a measure of blood oxygen transport during hypoxia exposure
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.rights.copyrightSelf-archiving of the author-manuscript version is not yet supported by this journal. Please refer to the journal link for access to the definitive, published version or contact the authors for more information.
gro.date.issued2012
gro.hasfulltextNo Full Text
gro.griffith.authorRenshaw, Gillian M.


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