Show simple item record

dc.contributor.authorWatts, Rebecca E.en_US
dc.contributor.authorTotsika, Makrinaen_US
dc.contributor.authorL. Challinor, Victoriaen_US
dc.contributor.authorN. Mabbett, Amadaen_US
dc.contributor.authorUlett, Glenen_US
dc.contributor.authorJ. De Voss, Jamesen_US
dc.contributor.authorA. Schembri, Marken_US
dc.date.accessioned2017-05-03T15:29:35Z
dc.date.available2017-05-03T15:29:35Z
dc.date.issued2012en_US
dc.date.modified2013-06-05T02:40:43Z
dc.identifier.issn00199567en_US
dc.identifier.doi10.1128/IAI.05594-11en_US
dc.identifier.urihttp://hdl.handle.net/10072/47641
dc.description.abstractThe molecular mechanisms that define asymptomatic bacteriuria (ABU) Escherichia coli colonization of the human urinary tract remain to be properly elucidated. Here, we utilize ABU E. coli strain 83972 as a model to dissect the contribution of siderophores to iron acquisition, growth, fitness, and colonization of the urinary tract. We show that E. coli 83972 produces enterobactin, salmochelin, aerobactin, and yersiniabactin and examine the role of these systems using mutants defective in siderophore biosynthesis and uptake. Enterobactin and aerobactin contributed most to total siderophore activity and growth in defined irondeficient medium. No siderophores were detected in an 83972 quadruple mutant deficient in all four siderophore biosynthesis pathways; this mutant did not grow in defined iron-deficient medium but grew in iron-limited pooled human urine due to iron uptake via the FecA ferric citrate receptor. In a mixed 1:1 growth assay with strain 83972, there was no fitness disadvantage of the 83972 quadruple biosynthetic mutant, demonstrating its capacity to act as a "cheater" and utilize siderophores produced by the wild-type strain for iron uptake. An 83972 enterobactin/salmochelin double receptor mutant was outcompeted by 83972 in human urine and the mouse urinary tract, indicating a role for catecholate receptors in urinary tract colonization.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_US
dc.languageEnglishen_US
dc.language.isoen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.publisher.placeUnited Statesen_US
dc.relation.ispartofstudentpublicationYen_US
dc.relation.ispartofpagefrom333en_US
dc.relation.ispartofpageto344en_US
dc.relation.ispartofissue1en_US
dc.relation.ispartofjournalInfection and Immunityen_US
dc.relation.ispartofvolume80en_US
dc.rights.retentionYen_US
dc.subject.fieldofresearchMedical Bacteriologyen_US
dc.subject.fieldofresearchcode110801en_US
dc.titleContribution of Siderophore Systems to Growth and Urinary Tract Colonization of Asymptomatic Bacteriuria Escherichia colien_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.date.issued2012
gro.hasfulltextNo Full Text


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record