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dc.contributor.authorIeva, Raffaele
dc.contributor.authorRoncarati, Davide
dc.contributor.authorMetruccio, Matteo ME
dc.contributor.authorSeib, Kate L
dc.contributor.authorScarlato, Vincenzo
dc.contributor.authorDelany, Isabel
dc.date.accessioned2017-05-03T15:50:52Z
dc.date.available2017-05-03T15:50:52Z
dc.date.issued2008
dc.date.modified2013-06-18T00:50:24Z
dc.identifier.issn0950-382X
dc.identifier.doi10.1111/j.1365-2958.2008.06468.x
dc.identifier.urihttp://hdl.handle.net/10072/47951
dc.description.abstractMechanisms for coping with oxidative stress (OS) are crucial for the survival of pathogenic Neisseria spp. in the human host. In this study we investigate the mechanism by which OxyR finely regulates the catalase gene (kat) in Neisseria meningitidis. Detailed transcriptional analyses show that catalase is transcribed from a single promoter that is induced by H2O2 in an OxyR-dependent manner and two key cysteine residues are essential for this. OxyR also represses the kat promoter: kat expression in the null mutant is at a constitutive intermediary level higher than uninduced, but lower than H2O2-induced levels in the wild type. Our data are consistent with a model in which OxyR binds to the kat promoter and exerts: (i) repression of transcription in the absence of OS signal and (ii) activation of the promoter in response to OS signal. This direct double-edged mechanism may ensure tight regulatory control of kat expression ensuring catalase is synthesized only when needed. In addition, our results provide an explanation for the altered OS resistance phenotypes seen in Neisseria mutant strains where, paradoxically, the oxyR mutants are more resistant than the wild type in oxidative killing assays.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.format.extent1631865 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoeng
dc.publisherWiley-Blackwell Publishing Ltd.
dc.publisher.placeUnited Kingdom
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom1152
dc.relation.ispartofpageto1165
dc.relation.ispartofissue5
dc.relation.ispartofjournalMolecular Microbiology
dc.relation.ispartofvolume70
dc.rights.retentionY
dc.subject.fieldofresearchBiological sciences
dc.subject.fieldofresearchBacteriology
dc.subject.fieldofresearchAgricultural, veterinary and food sciences
dc.subject.fieldofresearchBiomedical and clinical sciences
dc.subject.fieldofresearchcode31
dc.subject.fieldofresearchcode310701
dc.subject.fieldofresearchcode30
dc.subject.fieldofresearchcode32
dc.titleOxyR tightly regulates catalase expression in Neisseria meningitidis through both repression and activation mechanisms
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.rights.copyright© 2008 Blackwell Publishing Ltd. This is the pre-peer reviewed version of the following article: OxyR tightly regulates catalase expression in Neisseria meningitidis through both repression and activation mechanisms , Molecular Microbiology, Vol. 70(5), 2008, pp. 1152-1165, which has been published in final form at http://dx.doi.org/10.1111/j.1365-2958.2008.06468.x.
gro.date.issued2008
gro.hasfulltextFull Text
gro.griffith.authorSeib, Kate


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