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dc.contributor.authorTajouri, Lotfi
dc.contributor.authorMellick, Albert
dc.contributor.authorTourtellotte, A.
dc.contributor.authorNagra, R.
dc.contributor.authorGriffiths, Lyn
dc.date.accessioned2017-05-03T14:06:25Z
dc.date.available2017-05-03T14:06:25Z
dc.date.issued2005
dc.date.modified2008-11-30T22:45:46Z
dc.identifier.issn1385299X
dc.identifier.doi10.1016/j.brainresprot.2005.04.003
dc.identifier.urihttp://hdl.handle.net/10072/4858
dc.description.abstractWe have developed methods in real time detection of quantitative-polymerase chain reaction (Q-PCR) to analyse the relative levels of gene expression in post mortem brain tissue. We have then examined differences in gene activity between normal white matter (NWM) and plaque tissue from multiple sclerosis (MS) patients, displaying different pathologies (either acute or chronic active). The result from each MS plaque was then compared with an age and sex matched NWM control, obtained from the same anatomical location. The results of comparative and absolute Q-PCR analysis compared favourably (P<0.05, Pearsons), with significant differences in gene expression between chronic active and acute pathologies identified for four of the five genes examined. A 20-60-fold increase in osteopontin (SPP1) and inositol 1-4-5 phosphate 3 kinase B (ITPKB) levels was identified in acute plaques compared with NWM controls. This contrasted with a 5-fold (or less) increase in expression in chronic active plaques (P<0.05, unpaired t-Test). Both the signal transducer and activator of transcription (STAT)1 and the protein inhibitor of activated STAT1 (PIAS) displayed (in contrast) a 5-10 fold decrease in expression in acute tissues, with no significant difference in chronic active tissues, compared with NWM. Additionally, no association was identified with changes in Calpain (CAPNS1) levels and MS pathology. In summary, Q-PCR analysis has allowed the identification of clinically significant associations between gene expression and pathology from small amounts of CNS tissue; which may be used as the basis for further clinical investigations.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.publisher.placeNetherlands
dc.publisher.urihttp://www.elsevier.com/wps/find/journaldescription.cws_home/622287/description#description
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom79
dc.relation.ispartofpageto91
dc.relation.ispartofissue2
dc.relation.ispartofjournalBrain Research Protocols
dc.relation.ispartofvolume15
dc.rights.retentionY
dc.subject.fieldofresearchNeurosciences
dc.subject.fieldofresearchPsychology
dc.subject.fieldofresearchCognitive Sciences
dc.subject.fieldofresearchcode1109
dc.subject.fieldofresearchcode1701
dc.subject.fieldofresearchcode1702
dc.titleAn examination of MS candidate genes identified as differentially regulated in multiple sclerosis plaque tissue, using absolute and comparative real-time Q-PCR analysis.
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.facultyGriffith Health, School of Medicine
gro.rights.copyright© 2005 Elsevier : Reproduced in accordance with the copyright policy of the publisher : This journal is available online - use hypertext links.
gro.date.issued2005
gro.hasfulltextNo Full Text
gro.griffith.authorTajouri, Lotfi
gro.griffith.authorGriffiths, Lyn
gro.griffith.authorMellick, Albert S.


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