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dc.contributor.authorShum, Wai-Kei
dc.contributor.authorMaleknia, Simin
dc.contributor.authorM. Downard, Kevin
dc.date.accessioned2017-05-03T11:10:11Z
dc.date.available2017-05-03T11:10:11Z
dc.date.issued2005
dc.identifier.issn00032697
dc.identifier.doi10.1016/j.ab.2005.06.035
dc.identifier.urihttp://hdl.handle.net/10072/4897
dc.description.abstractThe early onset oxidative damage within segments of the protein a-crystallin is examined by radical probe mass spectrometry by its treatment with reactive oxygen species under low-, moderate-, and high-oxidizing conditions. Five regions comprising the first 11 residues of the N-termini of the A and B subunits (A/B:1-11), central domains from each subunit B:57-69 and A:104-112, and a C-terminal segment of the A subunit A:120-145 were found to be the initial sites of oxidation. The susceptibility of each segment to oxidation and oxidative damage is investigated by subjecting the intact protein to different oxidation conditions within the ion source of an electrospray ionization mass spectrometer. LC-MS of the oxidized protein digests enables the sites and levels of oxidation to be monitored. The onset of oxidative damage and the levels of oxidation observed before damage occurs differ across the protein surface. The regions comprising residues A/B:1-11 and A:104-112 are shown to be more susceptible to oxidative damage than those comprising residues B:57-69 and A:120-145. The results are discussed in the context of available experimental and homology-modeled theoretical structures for the subunits of a-crystallin.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherAcademic Press
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom247
dc.relation.ispartofpageto256
dc.relation.ispartofjournalAnalytical Biochemistry
dc.relation.ispartofvolume344
dc.rights.retentionN
dc.subject.fieldofresearchAnalytical Chemistry
dc.subject.fieldofresearchOther Chemical Sciences
dc.subject.fieldofresearchBiochemistry and Cell Biology
dc.subject.fieldofresearchcode0301
dc.subject.fieldofresearchcode0399
dc.subject.fieldofresearchcode0601
dc.titleOnset of oxidative damage in a-crystallin by radical probe mass spectrometry
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2015-02-06T01:36:45Z
gro.hasfulltextNo Full Text
gro.griffith.authorMaleknia, Simin


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