Show simple item record

dc.contributor.convenorBone-Tec Congresses and Symposia
dc.contributor.authorMuniz Renno, Ana Claudia
dc.contributor.authorMcDonnell, Pauline Ann
dc.contributor.authorCrovace, Murilo Camuri
dc.contributor.authorZanotto, Edgar Dutra
dc.contributor.authorLaakso, Liisa
dc.contributor.editorMikos, Antonios G.
dc.contributor.editorJohnson, Peter C.
dc.date.accessioned2017-05-03T12:57:51Z
dc.date.available2017-05-03T12:57:51Z
dc.date.issued2010
dc.date.modified2013-01-15T01:10:13Z
dc.identifier.issn1549-5418
dc.identifier.doi10.1089/pho.2009.2487
dc.identifier.urihttp://hdl.handle.net/10072/48982
dc.description.abstractThe purpose of this study was to develop a method for successfully seeding osteoblasts onto a glass-ceramic scaffold designed for use in clinical settings; and, determine whether the application of laser phototherapy at 830?nm would result in osteoblast proliferation on the glass-ceramic scaffold. The use of bioscaffolds is considered a promising strategy in a number of clinical applications where tissue healing is sub-optimal. As in vitro osteoblast growth is a slow process, laser phototherapy could be used to stimulate osteoblast proliferation on bioscaffolds. A methodology was developed to seed an osteoblastic (MC3T3) cell line on to a novel glass-ceramic scaffold. Seeded scaffolds were irradiated with a single exposure of 830?nm laser at 10?J/cm2 (at diode). Non-irradiated seeded scaffolds acted as negative controls. Cell proliferation was assessed 7 days after irradiation. Osteoblastic MC3T3 cells were successfully grown on discs composed of a glass-ceramic composite. Laser irradiation produced a 13% decrease in MC3T3 cell proliferation on glass-ceramic discs (mean+/-SD?=?0.192+/-0.002) compared to control (non-irradiated) discs (mean+/-SD?=?0.22+/-0.002). Despite successful seeding of bioscaffolds with osteoblasts, laser phototherapy resulted in a reduction in cell growth compared to non-irradiated controls. Future research combining laser phototherapy and glass-ceramic scaffolds should take in to account possible interactions of the laser with matrix compounds.
dc.description.publicationstatusYes
dc.format.extent56087 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.publisherMary Ann Liebert, Inc. Publishers
dc.publisher.placeGermany
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofconferencenameInternational Bone-Tissue-Engineering Congress
dc.relation.ispartofconferencetitlePHOTOMEDICINE AND LASER SURGERY
dc.relation.ispartofdatefrom2009-10-08
dc.relation.ispartofdateto2009-10-11
dc.relation.ispartoflocationUnited States
dc.relation.ispartofpagefrom131
dc.relation.ispartofpagefrom3 pages
dc.relation.ispartofpageto133
dc.relation.ispartofpageto3 pages
dc.relation.ispartofissue1
dc.relation.ispartofvolume28
dc.rights.retentionN
dc.subject.fieldofresearchClinical sciences
dc.subject.fieldofresearchClinical sciences not elsewhere classified
dc.subject.fieldofresearchcode3202
dc.subject.fieldofresearchcode320299
dc.titleEffect of 830nm Laser Phototherapy on Osteoblasts Grown in Vitro on Biosilicate Scaffolds
dc.typeConference output
dc.type.descriptionE3 - Conferences (Extract Paper)
dc.type.codeE - Conference Publications
gro.rights.copyrightThis is a copy of an article published in the Photomedicine and Laser Surgery. Copyright 2010 Mary Ann Liebert, Inc. Photomedicine and Laser Surgery is available online at: http://www.liebertonline.com
gro.date.issued2010
gro.hasfulltextFull Text
gro.griffith.authorMcDonnell, Ann A.
gro.griffith.authorLaakso, Liisa


Files in this item

This item appears in the following Collection(s)

  • Conference outputs
    Contains papers delivered by Griffith authors at national and international conferences.

Show simple item record