dc.contributor.convenor | Bone-Tec Congresses and Symposia | |
dc.contributor.author | Muniz Renno, Ana Claudia | |
dc.contributor.author | McDonnell, Pauline Ann | |
dc.contributor.author | Crovace, Murilo Camuri | |
dc.contributor.author | Zanotto, Edgar Dutra | |
dc.contributor.author | Laakso, Liisa | |
dc.contributor.editor | Mikos, Antonios G. | |
dc.contributor.editor | Johnson, Peter C. | |
dc.date.accessioned | 2017-05-03T12:57:51Z | |
dc.date.available | 2017-05-03T12:57:51Z | |
dc.date.issued | 2010 | |
dc.date.modified | 2013-01-15T01:10:13Z | |
dc.identifier.issn | 1549-5418 | |
dc.identifier.doi | 10.1089/pho.2009.2487 | |
dc.identifier.uri | http://hdl.handle.net/10072/48982 | |
dc.description.abstract | The purpose of this study was to develop a method for successfully seeding osteoblasts onto a glass-ceramic scaffold designed for use in clinical settings; and, determine whether the application of laser phototherapy at 830?nm would result in osteoblast proliferation on the glass-ceramic scaffold. The use of bioscaffolds is considered a promising strategy in a number of clinical applications where tissue healing is sub-optimal. As in vitro osteoblast growth is a slow process, laser phototherapy could be used to stimulate osteoblast proliferation on bioscaffolds. A methodology was developed to seed an osteoblastic (MC3T3) cell line on to a novel glass-ceramic scaffold. Seeded scaffolds were irradiated with a single exposure of 830?nm laser at 10?J/cm2 (at diode). Non-irradiated seeded scaffolds acted as negative controls. Cell proliferation was assessed 7 days after irradiation. Osteoblastic MC3T3 cells were successfully grown on discs composed of a glass-ceramic composite. Laser irradiation produced a 13% decrease in MC3T3 cell proliferation on glass-ceramic discs (mean+/-SD?=?0.192+/-0.002) compared to control (non-irradiated) discs (mean+/-SD?=?0.22+/-0.002). Despite successful seeding of bioscaffolds with osteoblasts, laser phototherapy resulted in a reduction in cell growth compared to non-irradiated controls. Future research combining laser phototherapy and glass-ceramic scaffolds should take in to account possible interactions of the laser with matrix compounds. | |
dc.description.publicationstatus | Yes | |
dc.format.extent | 56087 bytes | |
dc.format.mimetype | application/pdf | |
dc.language | English | |
dc.publisher | Mary Ann Liebert, Inc. Publishers | |
dc.publisher.place | Germany | |
dc.relation.ispartofstudentpublication | N | |
dc.relation.ispartofconferencename | International Bone-Tissue-Engineering Congress | |
dc.relation.ispartofconferencetitle | PHOTOMEDICINE AND LASER SURGERY | |
dc.relation.ispartofdatefrom | 2009-10-08 | |
dc.relation.ispartofdateto | 2009-10-11 | |
dc.relation.ispartoflocation | United States | |
dc.relation.ispartofpagefrom | 131 | |
dc.relation.ispartofpagefrom | 3 pages | |
dc.relation.ispartofpageto | 133 | |
dc.relation.ispartofpageto | 3 pages | |
dc.relation.ispartofissue | 1 | |
dc.relation.ispartofvolume | 28 | |
dc.rights.retention | N | |
dc.subject.fieldofresearch | Clinical sciences | |
dc.subject.fieldofresearch | Clinical sciences not elsewhere classified | |
dc.subject.fieldofresearchcode | 3202 | |
dc.subject.fieldofresearchcode | 320299 | |
dc.title | Effect of 830nm Laser Phototherapy on Osteoblasts Grown in Vitro on Biosilicate Scaffolds | |
dc.type | Conference output | |
dc.type.description | E3 - Conferences (Extract Paper) | |
dc.type.code | E - Conference Publications | |
gro.rights.copyright | This is a copy of an article published in the Photomedicine and Laser Surgery. Copyright 2010 Mary Ann Liebert, Inc. Photomedicine and Laser Surgery is available online at: http://www.liebertonline.com | |
gro.date.issued | 2010 | |
gro.hasfulltext | Full Text | |
gro.griffith.author | McDonnell, Ann A. | |
gro.griffith.author | Laakso, Liisa | |