Expression, purification and preliminary crystallographic analysis of sucrose phosphate synthase (SPS) from Halothermothrix orenii. .

Abstract

This is the first report of the crystallization of a sucrose phosphate synthase (SPS; EC 2.4.1.14). It also constitutes the first study of a sucrose phosphate synthase from a non-photosynthetic thermohalophilic anaerobic bacterium, Halothermothrix orenii. The purified recombinant spsA protein has been crystallized in the monoclinic space group C2, with unit-cell parameters a = 154.2, b = 47.9, c = 72.3 Ŭ = 103.16ଠusing the hanging-drop vapour-diffusion method. The crystal diffracts X-rays to a resolution limit of 3.01 Ů Heavy-metal and halide-soaking trials are currently in progress to solve the structure.