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dc.contributor.authorMaleknia, Simin
dc.contributor.authorM. Downard, Kevin
dc.date.accessioned2017-05-03T11:10:11Z
dc.date.available2017-05-03T11:10:11Z
dc.date.issued2005
dc.identifier.issn00032700
dc.identifier.doi10.1021/ac048961+
dc.identifier.urihttp://hdl.handle.net/10072/4921
dc.description.abstractA new method to interpret electrospray mass spectral data based on calculating the ratio of mass-to-charge (m/z) values of multiply charged ions is described. The mass-to-charge ratios of any two multiply charged ions corresponding to a single compound are unique numbers that enable the charge states for each ion to be unequivocally identified. The multiply charged ions in electrospray mass spectra originate from the addition or abstraction of protons, cations, or anions to and from a compound under analysis. In contrast to existing deconvolution processes, the charge ratio analysis method (CRAM), identifies the charge states of multiply charged ions without any prior knowledge of the nature of the charge-carrying species. In the case of high-resolution electrospray mass spectral data, in which multiply charged ions are resolved to their isotopic components, the CRAM is capable of correlating the isotope peaks of different multiply charged ions that share the same isotopic composition. This relative ratio method is illustrated here for electrospray mass spectral data of lysozyme and oxidized ubiquitin recorded at low- to high-mass resolution on quadrupole ion trap and Fourier transform ion cyclotron mass spectrometers, and theoretical data for the protein calmodulin based upon a reported spectrum recorded on the latter.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherAmerican Chemical Society
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom111
dc.relation.ispartofpageto119
dc.relation.ispartofissue1
dc.relation.ispartofjournalAnalytical Chemistry
dc.relation.ispartofvolume77
dc.rights.retentionN
dc.subject.fieldofresearchAnalytical Chemistry
dc.subject.fieldofresearchOther Chemical Sciences
dc.subject.fieldofresearchcode0301
dc.subject.fieldofresearchcode0399
dc.titleCharge ratio analysis method: approach for the deconvolution of electospray mass spectra
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.rights.copyrightSelf-archiving of the author-manuscript version is not yet supported by this journal. Please refer to the journal link for access to the definitive, published version or contact the author[s] for more information.
gro.date.issued2015-02-06T01:36:05Z
gro.hasfulltextNo Full Text
gro.griffith.authorMaleknia, Simin


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