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  • Quantification of vitellogenin mRNA induction in mosquitofish (Gambusia affinis) by reverse transcription real-time polymerase chain reaction (RT-PCR)

    Author(s)
    Leusch, FDL
    Van den Heuvel, MR
    Laurie, AD
    Chapman, HF
    Gooneratne, SR
    Tremblay, LA
    Griffith University Author(s)
    Leusch, Frederic
    Year published
    2005
    Metadata
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    Abstract
    A method to quantify induction of vitellogenin (Vtg) mRNA in adult male mosquitofish was developed. Male mosquitofish were exposed to 0, 1, 20 and 250 ng l-1 17ɿ-oestradiol (E2) for 4 and 8 days in static exposures, and liver Vtg mRNA and 18S rRNA expression were quantified in duplex RT-PCR. Liver 18S rRNA expression was very consistent among individuals, and there was a highly significant increase in Vtg mRNA expression after exposure of mosquitofish for just 4 days at 250 ng l-1 E2. Lower doses did not induce Vtg mRNA expression even at 4 or 8 days. This method could be used as a rapid test to detect exposure of mosquitofish ...
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    A method to quantify induction of vitellogenin (Vtg) mRNA in adult male mosquitofish was developed. Male mosquitofish were exposed to 0, 1, 20 and 250 ng l-1 17ɿ-oestradiol (E2) for 4 and 8 days in static exposures, and liver Vtg mRNA and 18S rRNA expression were quantified in duplex RT-PCR. Liver 18S rRNA expression was very consistent among individuals, and there was a highly significant increase in Vtg mRNA expression after exposure of mosquitofish for just 4 days at 250 ng l-1 E2. Lower doses did not induce Vtg mRNA expression even at 4 or 8 days. This method could be used as a rapid test to detect exposure of mosquitofish to oestrogenic chemicals. Further work is needed to determine if increased Vtg mRNA levels in male mosquitofish induce Vtg synthesis, and to determine the usefulness of the method in field sampling.
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    Journal Title
    Biomarkers
    Volume
    10
    Issue
    6
    DOI
    https://doi.org/10.1080/13547500500343381
    Copyright Statement
    © 2005 Taylor & Francis : The author-version of this article will be available for download [12-18 months] after publication : Use hypertext link to access the version of the publisher.
    Subject
    Environmental biotechnology
    Publication URI
    http://hdl.handle.net/10072/4951
    Collection
    • Journal articles

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