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dc.contributor.authorCase, Peter D
dc.contributor.authorBird, Philip S
dc.contributor.authorKahler, William A
dc.contributor.authorGeorge, Roy
dc.contributor.authorWalsh, Laurence J
dc.date.accessioned2017-05-03T15:34:30Z
dc.date.available2017-05-03T15:34:30Z
dc.date.issued2012
dc.date.modified2013-03-20T22:51:35Z
dc.identifier.issn0099-2399
dc.identifier.doi10.1016/j.joen.2011.12.020
dc.identifier.urihttp://hdl.handle.net/10072/49609
dc.description.abstractAbstract Introduction: Biofilms of resistant species such as Enterococcus faecalis pose a major challenge in the treatment of root canals with established periapical disease. This study examined the effects of gaseous ozone delivered into saline on biofilms of E. faecalis in root canals of extracted teeth with and without the use of passive ultrasonic agitation. Methods: Biofilms of E. faecalis were established over 14 days in 70 single roots that had undergone biomechanical preparation followed by gamma irradiation. The presence and purity of biofilms were confirmed using scanning electron microscopy and culture. Biofilms were treated with saline (negative control), 1% sodium hypochlorite for 120 seconds (positive control), ozone (140 ppm ozone in air at 2 L/min delivered into saline using a cannula for 120 seconds), saline with passive ultrasonic activation (70 kHz and 200 mW/cm2 applied to an ISO 15 file held passively within the canal, for 120 seconds), and ozone followed immediately by ultrasonic agitation. After treatment, samples were taken from the biofilm and serially diluted for plate counting. Results: Analysis revealed that 1% sodium hypochlorite was the most effective disinfecting agent followed by ozone combined with ultrasonic agitation, ozone alone, and finally ultrasonic alone. Conclusions: Although none of the treatment regimes were able to reliably render canals sterile under the conditions used, ozone gas delivered into irrigating fluids in the root canal may be useful as an adjunct for endodontic disinfection.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom523
dc.relation.ispartofpageto526
dc.relation.ispartofissue4
dc.relation.ispartofjournalJournal of Endodontics
dc.relation.ispartofvolume38
dc.rights.retentionY
dc.subject.fieldofresearchDentistry
dc.subject.fieldofresearchEndodontics
dc.subject.fieldofresearchcode3203
dc.subject.fieldofresearchcode320304
dc.titleTreatment of Root Canal Biofilms of Enterococcus faecalis with Ozone Gas and Passive Ultrasound Activation
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2012
gro.hasfulltextNo Full Text
gro.griffith.authorGeorge, Roy


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