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dc.contributor.authorMcRae, Shelleyen_US
dc.contributor.authorBrown, Chrisen_US
dc.contributor.authorBushell, Gillianen_US
dc.contributor.editorR.R. Burgessen_US
dc.date.accessioned2017-05-03T11:28:12Z
dc.date.available2017-05-03T11:28:12Z
dc.date.issued2005en_US
dc.date.modified2009-12-03T06:07:09Z
dc.identifier.issn10465928en_US
dc.identifier.doi10.1016/j.pep.2004.12.030en_AU
dc.identifier.urihttp://hdl.handle.net/10072/4975
dc.description.abstractMost current high throughput puriWcation procedures for the green Xuorescent protein (GFP) suVer from poor yields and low purity. An improved puriWcation procedure that delivers highly pure protein (>95% homogeneity) in high yields (>70% of the initial Xuorescent protein content) has been developed. The puriWcation procedure requires only two steps: the cell lysate is heated to 60 Í for 4min in ammonium sulfate and triethylamine, followed by hydrophobic interaction chromatography using isopropanol during the elution phase. The resulting pure product exhibits the same Xuorescence proWle as the crude sample. This procedure has been demonstrated on three commercial variants of GFP from Aequorea victoria, enhanced green, enhanced yellow, and enhanced cyan Xuorescent protein (Becton-Dickinson). The yield and purity of material are superior to other recently described methods.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_AU
dc.languageEnglishen_US
dc.language.isoen_AU
dc.publisherAcademic Pressen_US
dc.publisher.placeUSen_US
dc.publisher.urihttp://www.elsevier.com/wps/find/journaldescription.cws_home/622935/description#descriptionen_AU
dc.relation.ispartofstudentpublicationYen_AU
dc.relation.ispartofpagefrom121en_US
dc.relation.ispartofpageto127en_US
dc.relation.ispartofjournalProtein Expression and Purifcationen_US
dc.relation.ispartofvolume41en_US
dc.rights.retentionYen_AU
dc.subject.fieldofresearchcode270899en_US
dc.titleRapid purification of EGFP, EYFP, and ECFP with high yield and purityen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.rights.copyrightCopyright 2005 Elsevier : Reproduced in accordance with the copyright policy of the publisher : This journal is available online - use hypertext links.en_AU
gro.date.issued2005
gro.hasfulltextNo Full Text


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