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dc.contributor.authorVaquette, C
dc.contributor.authorFan, W
dc.contributor.authorXiao, Y
dc.contributor.authorHamlet, S
dc.contributor.authorIvanovski, S
dc.contributor.authorHutmacher, DW
dc.date.accessioned2017-05-03T16:05:31Z
dc.date.available2017-05-03T16:05:31Z
dc.date.issued2012
dc.date.modified2013-03-25T22:16:03Z
dc.identifier.issn1932-6254
dc.identifier.doi10.1002/term.1586
dc.identifier.urihttp://hdl.handle.net/10072/49784
dc.description.abstractThis study describes the design of a biphasic scaffold composed of a Fused Deposition Modeling scaffold (bone compartment) and an electrospun membrane (periodontal compartment) for periodontal complex regeneration. This strategy combined osteoblast impregnated in the bone compartment and multiple periodontal ligament (PDL) cell sheets on the electrospun membrane. After 21 days in culture the osteoblasts deposited mineralized matrix in the bone compartment and the cell sheets formed of a PDL-like tissue. The scaffolds were placed onto a dentin block and implanted in a rodent subcutaneous model for 8 weeks. In the bone compartment a more intense alkaline phosphatase staining was observed with higher bone density as shown by lCT, when the scaffold contained osteoblasts. A thin mineralized cementum- like tissue was deposited on the dentin surface for the groups with multiple PDL cell sheets (as observed by H&E and Azan staining). These groups showed a better attachment of PDL-like tissues onto the dentin block compared with the scaffold without cell sheets. Immunohistochemistry revealed the presence of cementum protein 1 (CEMP1), a specific marker for cementum, at the interface between PDL and the dentine blocks. These results demonstrated that the combination of multiple PDL cell sheets and a biphasic scaffold allows the simultaneous delivery of the cells necessary for in vivo regeneration of alveolar bone, periodontal ligament and cementum.
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherJohn Wiley & Sons
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom3
dc.relation.ispartofpageto3
dc.relation.ispartofissueSuppl. 1
dc.relation.ispartofjournalJournal of Tissue Engineering and Regenerative Medicine
dc.relation.ispartofvolume6
dc.rights.retentionY
dc.subject.fieldofresearchPeriodontics
dc.subject.fieldofresearchBiomedical Engineering
dc.subject.fieldofresearchClinical Sciences
dc.subject.fieldofresearchMedical Physiology
dc.subject.fieldofresearchcode110508
dc.subject.fieldofresearchcode0903
dc.subject.fieldofresearchcode1103
dc.subject.fieldofresearchcode1116
dc.titleA biphasic scaffold for simultaneous alveolar bone and periodontal ligament regeneration
dc.typeJournal article
dc.type.descriptionC3 - Articles (Letter/ Note)
dc.type.codeC - Journal Articles
gro.facultyGriffith Health, School of Dentistry and Oral Health
gro.date.issued2012
gro.hasfulltextNo Full Text
gro.griffith.authorHamlet, Stephen
gro.griffith.authorIvanovski, Saso
gro.griffith.authorHutmacher, Dietmar W.
gro.griffith.authorVaquette, Cedryck


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