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dc.contributor.authorSaw, Yu-Ting
dc.contributor.authorYang, Junzheng
dc.contributor.authorNg, Shu-Kay
dc.contributor.authorLiu, Shubai
dc.contributor.authorSingh, Surendra
dc.contributor.authorSingh, Margit
dc.contributor.authorWelch, William R
dc.contributor.authorTsuda, Hiroshi
dc.contributor.authorFong, Wing-Ping
dc.contributor.authorThompson, David
dc.contributor.authorVasiliou, Vasilis
dc.contributor.authorBerkowitz, Ross S
dc.contributor.authorNg, Shu-Wing
dc.date.accessioned2017-05-03T14:20:39Z
dc.date.available2017-05-03T14:20:39Z
dc.date.issued2012
dc.date.modified2013-03-25T22:22:31Z
dc.identifier.issn1471-2407
dc.identifier.doi10.1186/1471-2407-12-329
dc.identifier.urihttp://hdl.handle.net/10072/49793
dc.description.abstractBackground Aldehyde dehydrogenases belong to a superfamily of detoxifying enzymes that protect cells from carcinogenic aldehydes. Of the superfamily, ALDH1A1 has gained most attention because current studies have shown that its expression is associated with human cancer stem cells. However, ALDH1A1 is only one of the 19 human ALDH subfamilies currently known. The purpose of the present study was to determine if the expression and activities of other major ALDH isozymes are associated with human ovarian cancer and ovarian cancer sphere cultures. Methods Immunohistochemistry was used to delineate ALDH isozyme localization in clinical ovarian tissues. Western Blot analyses were performed on lysates prepared from cancer cell lines and ovarian cancer spheres to confirm the immunohistochemistry findings. Quantitative reverse transcription-polymerase chain reactions were used to measure the mRNA expression levels. The Aldefluorssay was used to measure ALDH activity in cancer cells from the four tumor subtypes. Results Immunohistochemical staining showed significant overexpression of ALDH1A3, ALDH3A2, and ALDH7A1 isozymes in ovarian tumors relative to normal ovarian tissues. The expression and activity of ALDH1A1 is tumor type-dependent, as seen from immunohistochemisty, Western blot analysis, and the Aldefluorssay. The expression was elevated in the mucinous and endometrioid ovarian epithelial tumors than in serous and clear cell tumors. In some serous and most clear cell tumors, ALDH1A1 expression was found in the stromal fibroblasts. RNA expression of all studied ALDH isozymes also showed higher expression in endometrioid and mucinous tumors than in the serous and clear cell subtypes. The expression of ALDH enzymes showed tumor type-dependent induction in ovarian cancer cells growing as sphere suspensions in serum-free medium. Conclusions The results of our study indicate that ALDH enzyme expression and activity may be associated with specific cell types in ovarian tumor tissues and vary according to cell states. Elucidating the function of the ALDH isozymes in lineage differentiation and pathogenesis may have significant implications for ovarian cancer pathophysiology.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.format.extent2790791 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoeng
dc.publisherBioMed Central
dc.publisher.placeUnited Kingdom
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom239-1
dc.relation.ispartofpageto239-12
dc.relation.ispartofjournalBMC Cancer
dc.relation.ispartofvolume12
dc.rights.retentionY
dc.subject.fieldofresearchOncology and carcinogenesis
dc.subject.fieldofresearchcode3211
dc.titleCharacterization of aldehyde dehydrogenase isozymes in ovarian cancer tissues and sphere cultures
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://creativecommons.org/licenses/by/2.0
gro.description.notepublicPage numbers are not for citation purposes. Instead, this article has the unique article number of 239.
gro.rights.copyright© 2012 Saw et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
gro.date.issued2012
gro.hasfulltextFull Text
gro.griffith.authorNg, Shu Kay Angus


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