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dc.contributor.authorVaquette, C
dc.contributor.authorHamlet, S
dc.contributor.authorIvanovski, S
dc.contributor.authorHutmacher, DW
dc.date.accessioned2017-05-03T16:05:31Z
dc.date.available2017-05-03T16:05:31Z
dc.date.issued2012
dc.date.modified2013-03-25T22:26:54Z
dc.identifier.issn1932-6254
dc.identifier.doi10.1002/term.1586
dc.identifier.urihttp://hdl.handle.net/10072/49799
dc.description.abstractCalcium phosphates (CaP) obtained by biomineralisation in Simulated Boby Fluid have been used for decades to assess the mineralisation capability of biomaterials. Recently, they have been envisioned as potential agents to promote bone formation. In this study, we have fabricated and coated with calcium phosphate melt electrospun scaffolds whereby macropores permit adequate cell migration and nutrient transfer. We have systematically investigated the effect of coating and osteoinduction onto the response of ovine osteoblasts and we observed that the coating up-regulated alkaline phosphatase activity regardless of the in vitro culture conditions. Micro Computed Tomography revealed that only scaffolds cultured in an osteoinductive cocktail were capable of depositing mineralised matrix, and that CaP coated scaffolds were more efficient at promoting mineralisation. Theses scaffolds were subcutaneously implanted in athymic rats and this demonstrated that the osteoinduction was a pre-requisite for bone formation in this ectopic model. It showed that although the bone formation was not significantly different after 8 weeks, the CaP coated scaffolds were superior at inducing bone formation as evidenced by higher levels of mineralisation at earlier time points. This work demonstrated that CaP coating is not sufficient to induce bone formation; however the combination of osteoinduction and CaP coating resulted in earlier bone formation in an ectopic model.
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherJohn Wiley & Sons
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom32
dc.relation.ispartofpageto32
dc.relation.ispartofissueSuppl. 1
dc.relation.ispartofjournalJournal of Tissue Engineering and Regenerative Medicine
dc.relation.ispartofvolume6
dc.rights.retentionY
dc.subject.fieldofresearchBiomedical engineering
dc.subject.fieldofresearchClinical sciences
dc.subject.fieldofresearchPeriodontics
dc.subject.fieldofresearchMedical physiology
dc.subject.fieldofresearchcode4003
dc.subject.fieldofresearchcode3202
dc.subject.fieldofresearchcode320310
dc.subject.fieldofresearchcode3208
dc.titleMelt electrospun calcium phosphate coated scaffolds accelerate ectopic bone formation in the context of a long term cultured cellular construct
dc.typeJournal article
dc.type.descriptionC3 - Articles (Letter/ Note)
dc.type.codeC - Journal Articles
gro.facultyGriffith Health, School of Dentistry and Oral Health
gro.date.issued2012
gro.hasfulltextNo Full Text
gro.griffith.authorHamlet, Stephen


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