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dc.contributor.authorSchroder, Wayneen_US
dc.contributor.authorBushell, Gillianen_US
dc.contributor.authorSculley, Tomen_US
dc.date.accessioned2017-05-03T11:32:22Z
dc.date.available2017-05-03T11:32:22Z
dc.date.issued2005en_US
dc.date.modified2009-12-05T05:16:59Z
dc.identifier.issn08986568en_US
dc.identifier.doi10.1016/j.cellsig.2004.10.015en_AU
dc.identifier.urihttp://hdl.handle.net/10072/4979
dc.description.abstractThe orthologous proteins of the stress-activated protein kinase-interacting 1 (Sin1) family have been implicated in several different signal transduction pathways. In this study, we have investigated the function of the full-length human Sin1 protein and a C-terminally truncated isoform, Sin1a, which is produced by alternative splicing. Immunoblot analysis using an anti-Sin1 polyclonal antibody showed that full-length Sin1 and several smaller isoforms are widely expressed. Sin1 was demonstrated to bind to c-Jun N-terminal kinase (JNK) in vitro and in vivo, while no interaction with p38- or ERK1/2-family MAPKs was observed. The Sin1a isoform could also form a complex with JNK in vivo. Despite localizing in distinct compartments within the cell, both Sin1 and Sin1a co-localized with JNK, suggesting that the Sin1 proteins could recruit JNK. Over-expression of full-length Sin1 inhibited the activation of JNK by UV-C in DG75 cells, as well as basal JNK-activity in HEK293 cells. These data suggest that the human Sin1 proteins may act as scaffold molecules in the regulation of signaling by JNK.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_AU
dc.languageEnglishen_US
dc.language.isoen_AU
dc.publisherElsevieren_US
dc.publisher.placeUSAen_US
dc.publisher.urihttp://www.elsevier.com/wps/find/journaldescription.cws_home/525462/description#descriptionen_AU
dc.relation.ispartofstudentpublicationYen_AU
dc.relation.ispartofpagefrom761en_US
dc.relation.ispartofpageto767en_US
dc.relation.ispartofissue6en_US
dc.relation.ispartofjournalCellular Signallingen_US
dc.relation.ispartofvolume17en_US
dc.rights.retentionYen_AU
dc.subject.fieldofresearchcode270103en_US
dc.titleThe human stress-activated protein kinase-interacting 1 gene encodes JNK-binding proteins.en_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.rights.copyrightCopyright 2002 Elsevier : Reproduced in accordance with the copyright policy of the publisher : This journal is available online - use hypertext linksen_AU
gro.date.issued2005
gro.hasfulltextNo Full Text


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