dc.contributor.author | Stephens, Alexandre | |
dc.contributor.author | Day, Christopher | |
dc.contributor.author | Tiralongo, Joe | |
dc.date.accessioned | 2017-05-03T14:16:58Z | |
dc.date.available | 2017-05-03T14:16:58Z | |
dc.date.issued | 2012 | |
dc.date.modified | 2013-04-29T02:41:41Z | |
dc.identifier.issn | 23257776 | |
dc.identifier.doi | 10.4236/cellbio.2012.12005 | |
dc.identifier.uri | http://hdl.handle.net/10072/49845 | |
dc.description.abstract | Cell-based assays represent a major end point of high throughput screening (HTS) but a key limitation of such assays is the potentially poor membrane permeability of test compounds. In this study, we optimized the conditions for the deliv-ery of the membrane impermeable compound 8-hydroxypyrene-1,3,6-trisulfonic acid trisodium salt (HPTS) into human cells using hypotonic shift; a method that can promote the uptake of molecules from the extracellular fluid into cell cy-toplasm via endocytosis. We showed that uptake of HPTS by cells was a function of hypotonic buffer osmolarity and that delivery was highly efficient with almost 100% of cells displaying uptake. Delivery of HPTS was equally effective at 25àand 37ì with delivery of compound proportional to incubation time and concentration of HPTS within the loading medium. The experimental conditions identified in this study could be applied to HTS drug discovery studies providing an effective method of delivering small membrane impermeable compounds into cells. | |
dc.description.peerreviewed | Yes | |
dc.description.publicationstatus | Yes | |
dc.format.extent | 227770 bytes | |
dc.format.mimetype | application/pdf | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | Scientific Research | |
dc.publisher.place | United States | |
dc.relation.ispartofstudentpublication | N | |
dc.relation.ispartofpagefrom | 38 | |
dc.relation.ispartofpageto | 42 | |
dc.relation.ispartofjournal | CellBio | |
dc.relation.ispartofvolume | 1 | |
dc.rights.retention | Y | |
dc.subject.fieldofresearch | Receptors and Membrane Biology | |
dc.subject.fieldofresearch | Analytical Biochemistry | |
dc.subject.fieldofresearchcode | 060110 | |
dc.subject.fieldofresearchcode | 060101 | |
dc.title | Optimized Conditions for the Delivery of Small Membrane Impermeable Compounds into Human Cells Using Hypotonic Shift | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
gro.faculty | Griffith Health, School of Medical Science | |
gro.rights.copyright | © 2012 Scientific Research Publishing. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal website for access to the definitive, published version. | |
gro.date.issued | 2012 | |
gro.hasfulltext | Full Text | |
gro.griffith.author | Day, Christopher J. | |
gro.griffith.author | Stephens, Alexandre | |
gro.griffith.author | Tiralongo, Joe | |