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dc.contributor.authorStephens, Alexandre
dc.contributor.authorDay, Christopher
dc.contributor.authorTiralongo, Joe
dc.date.accessioned2017-05-03T14:16:58Z
dc.date.available2017-05-03T14:16:58Z
dc.date.issued2012
dc.date.modified2013-04-29T02:41:41Z
dc.identifier.issn23257776
dc.identifier.doi10.4236/cellbio.2012.12005
dc.identifier.urihttp://hdl.handle.net/10072/49845
dc.description.abstractCell-based assays represent a major end point of high throughput screening (HTS) but a key limitation of such assays is the potentially poor membrane permeability of test compounds. In this study, we optimized the conditions for the deliv-ery of the membrane impermeable compound 8-hydroxypyrene-1,3,6-trisulfonic acid trisodium salt (HPTS) into human cells using hypotonic shift; a method that can promote the uptake of molecules from the extracellular fluid into cell cy-toplasm via endocytosis. We showed that uptake of HPTS by cells was a function of hypotonic buffer osmolarity and that delivery was highly efficient with almost 100% of cells displaying uptake. Delivery of HPTS was equally effective at 25àand 37ì with delivery of compound proportional to incubation time and concentration of HPTS within the loading medium. The experimental conditions identified in this study could be applied to HTS drug discovery studies providing an effective method of delivering small membrane impermeable compounds into cells.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.format.extent227770 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoeng
dc.publisherScientific Research
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom38
dc.relation.ispartofpageto42
dc.relation.ispartofjournalCellBio
dc.relation.ispartofvolume1
dc.rights.retentionY
dc.subject.fieldofresearchReceptors and Membrane Biology
dc.subject.fieldofresearchAnalytical Biochemistry
dc.subject.fieldofresearchcode060110
dc.subject.fieldofresearchcode060101
dc.titleOptimized Conditions for the Delivery of Small Membrane Impermeable Compounds into Human Cells Using Hypotonic Shift
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.facultyGriffith Health, School of Medical Science
gro.rights.copyright© 2012 Scientific Research Publishing. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal website for access to the definitive, published version.
gro.date.issued2012
gro.hasfulltextFull Text
gro.griffith.authorDay, Christopher J.
gro.griffith.authorStephens, Alexandre
gro.griffith.authorTiralongo, Joe


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