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  • hnRNP A2, a Potential ssDNA/RNA Molecular Adapter at the Telomere

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    Author(s)
    Moran-Jones, K
    Wayman, L
    Kennedy, DD
    Reddel, RR
    Sara, S
    Snee, MJ
    Smith, R
    Griffith University Author(s)
    Kennedy, Derek D.
    Year published
    2005
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    Abstract
    The heterogeneous nuclear ribonucleoprotein (hnRNP) A2 is a multi-tasking protein that acts in the cytoplasm and nucleus. We have explored the possibility that this protein is associated with telomeres and participates in their maintenance. Rat brain hnRNP A2 was shown to have two nucleic acid binding sites. In the presence of heparin one site binds single-stranded oligodeoxyribonucleotides irrespective of sequence but not the corresponding oligoribonucleotides. Both the hnRNP A2-binding cis-acting element for the cytoplasmic RNA trafficking element, A2RE, and the ssDNA telomere repeat match a consensus sequence for binding ...
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    The heterogeneous nuclear ribonucleoprotein (hnRNP) A2 is a multi-tasking protein that acts in the cytoplasm and nucleus. We have explored the possibility that this protein is associated with telomeres and participates in their maintenance. Rat brain hnRNP A2 was shown to have two nucleic acid binding sites. In the presence of heparin one site binds single-stranded oligodeoxyribonucleotides irrespective of sequence but not the corresponding oligoribonucleotides. Both the hnRNP A2-binding cis-acting element for the cytoplasmic RNA trafficking element, A2RE, and the ssDNA telomere repeat match a consensus sequence for binding to a second sequence-specific site identified by mutational analysis. hnRNP A2 protected the telomeric repeat sequence, but not the complementary sequence, against DNase digestion: the glycine-rich domain was found to be necessary, but not sufficient, for protection. The N-terminal RRM (RNA recognition motif) and tandem RRMs of hnRNP A2 also bind the single-stranded, template-containing segment of telomerase RNA. hnRNP A2 colocalizes with telomeric chromatin in the subset of PML bodies that are a hallmark of ALT cells, reinforcing the evidence for hnRNPs having a role in telomere maintenance. Our results support a model in which hnRNP A2 acts as a molecular adapter between singlestranded telomeric repeats, or telomerase RNA, and another segment of ssDNA.
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    Journal Title
    Nucleic Acids Research
    Volume
    33
    Issue
    2
    Publisher URI
    http://nar.oxfordjournals.org/cgi/content/full/33/2/486
    DOI
    https://doi.org/10.1093/nar/gki203
    Copyright Statement
    © 2005 Moran-Jones et al. This article has been published under an open access model.
    Subject
    Environmental sciences
    Biological sciences
    Information and computing sciences
    Publication URI
    http://hdl.handle.net/10072/4985
    Collection
    • Journal articles

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