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dc.contributor.authorVargas, Sergio
dc.contributor.authorSchuster, Astrid
dc.contributor.authorSacher, Katharina
dc.contributor.authorBuettner, Gabrielle
dc.contributor.authorSchaetzle, Simone
dc.contributor.authorLaeuchli, Benjamin
dc.contributor.authorHall, Kathryn
dc.contributor.authorHooper, John NA
dc.contributor.authorErpenbeck, Dirk
dc.contributor.authorWoerheide, Gert
dc.date.accessioned2018-01-12T00:45:15Z
dc.date.available2018-01-12T00:45:15Z
dc.date.issued2012
dc.date.modified2013-06-27T00:03:31Z
dc.identifier.issn1932-6203
dc.identifier.doi10.1371/journal.pone.0039345
dc.identifier.urihttp://hdl.handle.net/10072/50502
dc.description.abstractBackground Phylum Porifera includes ~8,500 valid species distributed world-wide in aquatic ecosystems ranging from ephemeral fresh-water bodies to coastal environments and the deep-sea. The taxonomy and systematics of sponges is complicated, and morphological identification can be both time consuming and erroneous due to phenotypic convergence and secondary losses, etc. DNA barcoding can provide sponge biologists with a simple and rapid method for the identification of samples of unknown taxonomic membership. The Sponge Barcoding Project (www.spongebarcoding.org), the first initiative to barcode a non-bilaterian metazoan phylum, aims to provide a comprehensive DNA barcode database for Phylum Porifera. Methodology/Principal Findings ~7,400 sponge specimens have been extracted, and amplification of the standard COI barcoding fragment has been attempted for approximately 3,300 museum samples with ~25% mean amplification success. Based on this comprehensive sampling, we present the first report on the workflow and progress of the sponge barcoding project, and discuss some common pitfalls inherent to the barcoding of sponges. Conclusion A DNA-barcoding workflow capable of processing potentially large sponge collections has been developed and is routinely used for the Sponge Barcoding Project with success. Sponge specific problems such as the frequent co-amplification of non-target organisms have been detected and potential solutions are currently under development. The initial success of this innovative project have already demonstrated considerable refinement of sponge systematics, evaluating morphometric character importance, geographic phenotypic variability, and the utility of the standard barcoding fragment for Porifera (despite its conserved evolution within this basal metazoan phylum).
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoen_US
dc.publisherPublic Library of Science
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrome39345-1
dc.relation.ispartofpagetoe39345-7
dc.relation.ispartofissue7
dc.relation.ispartofjournalPloS One
dc.relation.ispartofvolume7
dc.rights.retentionY
dc.subject.fieldofresearchBiologically Active Molecules
dc.subject.fieldofresearchcode030401
dc.titleBarcoding Sponges: An Overview Based on Comprehensive Sampling
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttps://creativecommons.org/licenses/by/4.0/
dc.description.versionPublished
gro.rights.copyright© 2012 Vargas et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
gro.date.issued2012
gro.hasfulltextFull Text
gro.griffith.authorHooper, John N.


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