dc.contributor.author | Brown, NF | |
dc.contributor.author | Logue, CA | |
dc.contributor.author | Boddey, JA | |
dc.contributor.author | Scott, R | |
dc.contributor.author | Hirst, RG | |
dc.contributor.author | Beacham, IR | |
dc.contributor.editor | C. P. Hollenberg | |
dc.date.accessioned | 2017-05-03T11:14:44Z | |
dc.date.available | 2017-05-03T11:14:44Z | |
dc.date.issued | 2004 | |
dc.date.modified | 2010-08-06T07:21:26Z | |
dc.identifier.issn | 1617-4615 | |
dc.identifier.doi | 10.1007/s00438-004-1039-z | |
dc.identifier.uri | http://hdl.handle.net/10072/5057 | |
dc.description.abstract | Two adjacent genes, bpaA and bpaB, whose products display significant similarity to a number of two-partner secretion (TPS) systems have been identified in Burkholderia pseudomallei strain 08, but are absent from the closely related avirulent species B. thailandensis. They possess a number of sequence features characteristic of TPS systems, including the presence of an NPNGI motif in a region of BpaA which strongly resembles a TPS secretion domain. BpaA is a very large protein (~530 kDa) and contains three repeats, each 600-800-amino acids long. Putative membrane-spanning regions in BpaB were identified through alignment with TpsB family members, and this also revealed an N-terminal extension not found in other TpsB proteins. The bpaA gene was found to be absent from the majority of B. pseudomallei strains. It appears that bpaAB are located within a putative genomic island that is inserted in close proximity to a methionine tRNACAT-encoding gene. Expression of BpaA was undetectable in cells grown in laboratory media. However, owing to the similarity of BpaA to known adhesin molecules, a potential role of BpaA in virulence was investigated in cell culture and in an animal model, but no evidence for such a role was found in these test systems. | |
dc.description.peerreviewed | Yes | |
dc.description.publicationstatus | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | Springer-Verlag | |
dc.publisher.place | Berlin, Heidelberg | |
dc.relation.ispartofpagefrom | 204 | |
dc.relation.ispartofpageto | 215 | |
dc.relation.ispartofjournal | Molecular Genetics and Genomics | |
dc.relation.ispartofvolume | 272 | |
dc.subject.fieldofresearch | Genetics | |
dc.subject.fieldofresearch | Plant biology | |
dc.subject.fieldofresearchcode | 3105 | |
dc.subject.fieldofresearchcode | 3108 | |
dc.title | Identification of a novel two-partner secretion system from Burkholderia pseudomallei. | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
gro.faculty | Griffith Health, School of Medical Science | |
gro.date.issued | 2004 | |
gro.hasfulltext | No Full Text | |
gro.griffith.author | Beacham, Ifor R. | |