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dc.contributor.authorBrown, NF
dc.contributor.authorLogue, CA
dc.contributor.authorBoddey, JA
dc.contributor.authorScott, R
dc.contributor.authorHirst, RG
dc.contributor.authorBeacham, IR
dc.contributor.editorC. P. Hollenberg
dc.date.accessioned2017-05-03T11:14:44Z
dc.date.available2017-05-03T11:14:44Z
dc.date.issued2004
dc.date.modified2010-08-06T07:21:26Z
dc.identifier.issn1617-4615
dc.identifier.doi10.1007/s00438-004-1039-z
dc.identifier.urihttp://hdl.handle.net/10072/5057
dc.description.abstractTwo adjacent genes, bpaA and bpaB, whose products display significant similarity to a number of two-partner secretion (TPS) systems have been identified in Burkholderia pseudomallei strain 08, but are absent from the closely related avirulent species B. thailandensis. They possess a number of sequence features characteristic of TPS systems, including the presence of an NPNGI motif in a region of BpaA which strongly resembles a TPS secretion domain. BpaA is a very large protein (~530 kDa) and contains three repeats, each 600-800-amino acids long. Putative membrane-spanning regions in BpaB were identified through alignment with TpsB family members, and this also revealed an N-terminal extension not found in other TpsB proteins. The bpaA gene was found to be absent from the majority of B. pseudomallei strains. It appears that bpaAB are located within a putative genomic island that is inserted in close proximity to a methionine tRNACAT-encoding gene. Expression of BpaA was undetectable in cells grown in laboratory media. However, owing to the similarity of BpaA to known adhesin molecules, a potential role of BpaA in virulence was investigated in cell culture and in an animal model, but no evidence for such a role was found in these test systems.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherSpringer-Verlag
dc.publisher.placeBerlin, Heidelberg
dc.relation.ispartofpagefrom204
dc.relation.ispartofpageto215
dc.relation.ispartofjournalMolecular Genetics and Genomics
dc.relation.ispartofvolume272
dc.subject.fieldofresearchGenetics
dc.subject.fieldofresearchPlant biology
dc.subject.fieldofresearchcode3105
dc.subject.fieldofresearchcode3108
dc.titleIdentification of a novel two-partner secretion system from Burkholderia pseudomallei.
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.facultyGriffith Health, School of Medical Science
gro.date.issued2004
gro.hasfulltextNo Full Text
gro.griffith.authorBeacham, Ifor R.


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