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dc.contributor.authorPower, PM
dc.contributor.authorJones, RA
dc.contributor.authorBeacham, IR
dc.contributor.authorBucholtz, C
dc.contributor.authorJennings, MP
dc.contributor.editorW. Baumeister
dc.date.accessioned2017-05-03T11:14:45Z
dc.date.available2017-05-03T11:14:45Z
dc.date.issued2004
dc.date.modified2014-02-28T02:03:32Z
dc.identifier.issn0006-291X
dc.identifier.doi10.1016/j.bbrc.2004.08.022
dc.identifier.urihttp://hdl.handle.net/10072/5064
dc.description.abstractTranslational pausing may occur due to a number of mechanisms, including the presence of non-optimal codons, and it is thought to play a role in the folding of specific polypeptide domains during translation and in the facilitation of signal peptide recognition during sec-dependent protein targeting. In this whole genome analysis of Escherichia coli we have found that non-optimal codons in the signal peptide-encoding sequences of secretory genes are overrepresented relative to the "mature" portions of these genes; this is in addition to their overrepresentation in the 5'-regions of genes encoding non-secretory proteins. We also find increased non-optimal codon usage at the 3' ends of most E. coli genes, in both non-secretory and secretory sequences. Whereas presumptive translational pausing at the 5' and 3' ends of E. coli messenger RNAs may clearly have a general role in translation, we suggest that it also has a specific role in sec-dependent protein export, possibly in facilitating signal peptide recognition. This finding may have important implications for our understanding of how the majority of non-cytoplasmic proteins are targeted, a process that is essential to all biological cells.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoen_US
dc.publisherElsevier
dc.publisher.placeAmsterdam
dc.relation.ispartofpagefrom1038
dc.relation.ispartofpageto1044
dc.relation.ispartofjournalBiochemical and Biophysical Research Communications
dc.relation.ispartofvolume322
dc.subject.fieldofresearchMedicinal and Biomolecular Chemistry
dc.subject.fieldofresearchBiochemistry and Cell Biology
dc.subject.fieldofresearchMedical Biochemistry and Metabolomics
dc.subject.fieldofresearchcode0304
dc.subject.fieldofresearchcode0601
dc.subject.fieldofresearchcode1101
dc.titleWhole genome analysis reveals a high incidence of non-optimal codons in secretory signal sequences of Escherichia coli
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.rights.copyright© 2004 Elsevier : Reproduced in accordance with the copyright policy of the publisher : This journal is available online - use hypertext links.
gro.date.issued2004
gro.hasfulltextNo Full Text
gro.griffith.authorBeacham, Ifor R.
gro.griffith.authorJennings, Michael P.


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