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dc.contributor.authorCock, Ianen_US
dc.contributor.authorKalt, Freden_US
dc.date.accessioned2017-05-03T11:28:44Z
dc.date.available2017-05-03T11:28:44Z
dc.date.issued2012en_US
dc.date.modified2013-05-24T03:27:29Z
dc.identifier.issn22297715en_US
dc.identifier.doi10.4103/2229-5119.102749en_US
dc.identifier.urihttp://hdl.handle.net/10072/50843
dc.description.abstractBackground: Xanthorrhoea johnsonii A. T. Lee is a slow growing endemic Australian plant species. Its slow growth rate and lack of use by herbivores as a food source indicate that its leaves may contain chemical deterrents. Materials and Methods: X. johnsonii leaf extracts were tested for toxicity using the Artemia franciscana nauplii bioassay. Toxic extracts were further analysed by RP-HPLC and GC-MS. Results: As well as toxicity, anaesthetic/sedative effects were also noted in the methanolic and ethyl acetate extracts. A. franciscana nauplii 'died' following a 12 h exposure to these extracts, only to 'recover' and swim normally by 48 h exposure. By 60 h the X. johnsonii methanol/ethyl acetate extract exposed nauplii were again experiencing toxic effects and the mortality began to increase. The nauplii did not recover from this moribund state a second time. Analysis of these extracts by RP-HPLC showed that the ethyl acetate extract was the least complex of the toxic extracts. Subsequent analysis of this extract by GC-MS revealed that it contained 12 main compounds: o-hydroxycinnamic acid; acetic acid; 2,2-dimethoxybutane; pinacol; 4-methyl-1,3-dioxane; decane, 2,2-dimethylbutane; 2,5,9-trimethyldecane; 1,2-benzenediol; 1,2,3-benzenetriol; benzoic acid and an unidentified compound. Conclusion: These results confirm the toxicity and anaesthetic/sedative effects of X. johnsonii leaf components and highlight o-hydroxycinnamic acid as the likely compound responsible for this bioactivity.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_US
dc.format.extent1645327 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglishen_US
dc.publisherMedknow Publications and Mediaen_US
dc.publisher.placeIndiaen_US
dc.relation.ispartofstudentpublicationNen_US
dc.relation.ispartofpagefrom78en_US
dc.relation.ispartofpageto88en_US
dc.relation.ispartofissue2en_US
dc.relation.ispartofjournalJournal of Natural Pharmaceuticalsen_US
dc.relation.ispartofvolume3en_US
dc.rights.retentionYen_US
dc.subject.fieldofresearchPlant Biology not elsewhere classifieden_US
dc.subject.fieldofresearchcode060799en_US
dc.titleGas chromatography-mass spectroscopy analysis of a Xanthorrhoea johnsonii leaf extract displaying apparent anaesthetic effectsen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.facultyGriffith Sciences, School of Natural Sciencesen_US
gro.rights.copyright© 2012 Phcog.net. The attached file is reproduced here in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.en_US
gro.date.issued2012
gro.hasfulltextFull Text


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