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dc.contributor.authorOROZCO, C
dc.contributor.authorCOCK, I
dc.contributor.authorPERKINS, AV
dc.contributor.authorCLARKE, FM
dc.date.accessioned2012-03-19
dc.date.accessioned2013-05-31T01:02:02Z
dc.date.accessioned2017-03-01T22:29:33Z
dc.date.available2017-03-01T22:29:33Z
dc.date.issued1990
dc.date.modified2013-05-31T01:02:02Z
dc.identifier.issn0022-4251
dc.identifier.doi10.1530/jrf.0.0880447
dc.identifier.urihttp://hdl.handle.net/10072/50968
dc.description.abstractWhen male mouse spleen cells were incubated with a combination of platelet activating factor (PAF, 1-0-alkyl-2-acetyl-sn-glycero-3-phosphocholine) and sera from female mice in oestrus, the cells displayed a markedly increased rosette inhibition titre (RIT) when subsequently tested in the rosette inhibition assay. Neither PAF nor oestrous mouse sera alone could induce this effect, the combined action was required. Lyso-PAF could not substitute for the PAF, nor could male mouse sera nor the sera from females in dioestrus or metoestrus substitute for the oestrous mouse serum requirement. Pro-oestrous mouse sera could replace oestrous mouse sera but were less effective in their dose\p=n-\responses.Studies on the mechanism of action of the PAF and oestrous mouse serum components suggested that the PAF stimulated the production and release of soluble factors (termed S2 factors) which by themselves could induce increased RIT values when applied to fresh spleen cells. The PAF-stimulated cell populations were rendered refractory to the action of these S2 factors and did not display increased RIT values, unless oestrous mouse serum was added. This serum acted to reverse the refractory state, allowing the S2 factors to exert their effect, and so cells treated with PAF and oestrous mouse serum displayed increased RIT values.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherGreat Britain
dc.publisherThe Society for Reproduction and Fertility
dc.relation.ispartofpagefrom447
dc.relation.ispartofpageto457
dc.relation.ispartofjournalJournal of Reproduction and Fertility
dc.relation.ispartofvolume88
dc.subject.fieldofresearchBiological Sciences not elsewhere classified
dc.subject.fieldofresearchPhysiology
dc.subject.fieldofresearchClinical Sciences
dc.subject.fieldofresearchPaediatrics and Reproductive Medicine
dc.subject.fieldofresearchcode069999
dc.subject.fieldofresearchcode0606
dc.subject.fieldofresearchcode1103
dc.subject.fieldofresearchcode1114
dc.titlePlatelet-activating factor and serum components from oestrous mice co-operate to mimic the activity of 'early pregnancy factor' in the rosette inhibition assay
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codec1
gro.facultyFaculty of Science, Environment, Engineering and Technology
gro.date.issued1990
gro.hasfulltextNo Full Text
gro.griffith.authorClarke, Francis M.
gro.griffith.authorPerkins, Anthony V.
gro.griffith.authorCock, Ian E.
gro.griffith.authorOrozco, Carlos


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