Show simple item record

dc.contributor.authorSemchenko, Evgeny A
dc.contributor.authorDay, Christopher J
dc.contributor.authorMoutin, Marc
dc.contributor.authorWilson, Jennifer C
dc.contributor.authorTiralongo, Joe
dc.contributor.authorKorolik, Victoria
dc.contributor.editorDamien Pattinson
dc.date.accessioned2017-05-03T12:19:08Z
dc.date.available2017-05-03T12:19:08Z
dc.date.issued2012
dc.date.modified2013-06-21T03:46:21Z
dc.identifier.issn1932-6203
dc.identifier.doi10.1371/journal.pone.0040920
dc.identifier.urihttp://hdl.handle.net/10072/51804
dc.description.abstractLipooligosaccharides of the gastrointestinal pathogen Campylobacter jejuni are regarded as a major virulence factor and are implicated in the production of cross-reactive antibodies against host gangliosides, which leads to the development of autoimmune neuropathies such as Guillain-Barre 䠡nd Fisher Syndromes. C. jejuni strains are known to produce diverse LOS structures encoded by more than 19 types of LOS biosynthesis clusters. This study demonstrates that the final C. jejuni LOS structure cannot always be predicted from the genetic composition of the LOS biosynthesis cluster, as determined by novel lectin array analysis of the terminal LOS glycans. The differences were shown to be partially facilitated by the differential on/ off status of three genes wlaN, cst and cj1144-45. The on/off status of these genes was also analysed in C. jejuni strains grown in vitro and in vivo, isolated directly from the host animal without passaging, using immunoseparation. Importantly, C. jejuni strains 331, 421 and 520 encoding cluster type C were shown to produce different LOS, mimicking asialo GM1, asialo GM2 and a heterogeneous mix of gangliosides and other glycoconjugates respectively. In addition, individual C. jejuni colonies were shown to consistently produce heterogeneous LOS structures, irrespective of the cluster type and the status of phase variable genes. Furthermore we describe C. jejuni strains (351 and 375) with LOS clusters that do not match any of the previously described LOS clusters, yet are able to produce LOS with asialo GM2-like mimicries. The LOS biosynthesis clusters of these strains are likely to contain genes that code for LOS biosynthesis machinery previously not identified, yet capable of synthesising LOS mimicking gangliosides.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.format.extent733563 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoeng
dc.publisherPublic Library of Science
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrome40920-1
dc.relation.ispartofpagetoe40920-11
dc.relation.ispartofissue7
dc.relation.ispartofjournalPloS One
dc.relation.ispartofvolume7
dc.rights.retentionY
dc.subject.fieldofresearchMicrobial genetics
dc.subject.fieldofresearchcode310704
dc.titleStructural Heterogeneity of Terminal Glycans in Campylobacter jejuni Lipooligosaccharides
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://www.plos.org/journals/license.html
gro.facultyGriffith Sciences, School of Natural Sciences
gro.rights.copyright© 2012 Semchenko et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License CCAL. (http://www.plos.org/journals/license.html)
gro.date.issued2012
gro.hasfulltextFull Text
gro.griffith.authorKorolik, Victoria
gro.griffith.authorDay, Christopher J.
gro.griffith.authorTiralongo, Joe
gro.griffith.authorSemchenko, Evgeny


Files in this item

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record