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dc.contributor.authorTomasetti, Marco
dc.contributor.authorStaffolani, Sara
dc.contributor.authorNocchi, Linda
dc.contributor.authorNeuzil, Jiri
dc.contributor.authorStrafella, Elisabetta
dc.contributor.authorManzella, Nicola
dc.contributor.authorMariotti, Laura
dc.contributor.authorBracci, Massimo
dc.contributor.authorMatteo, Valentino A
dc.contributor.authorAmati, Monica
dc.contributor.authorSantarelli, Lory
dc.date.accessioned2017-05-03T12:22:13Z
dc.date.available2017-05-03T12:22:13Z
dc.date.issued2012
dc.date.modified2013-06-21T03:52:18Z
dc.identifier.issn0009-9120
dc.identifier.doi10.1016/j.clinbiochem.2012.02.009
dc.identifier.urihttp://hdl.handle.net/10072/51809
dc.description.abstractObjectives: Aim of this study was to evaluate the accuracy and precision of the detection of individual miRNA as clinical biomarkers in the serum. Design and methods: miRNA-126 was quantified in serum using endogenous and exogenous controls fornormalization and the accuracy and precision of the method evaluated. The diagnostic value of serummiRNA-126 was evaluated in malignant esothelioma (MM) and non-small-cell lung cancer (NSCLC) patients using both relative and absolute qRT-PCR methods. Results: The use of endogenous invariant and exogenous synthetic controls as well sample dilution markedly improves the accuracy and precision of the assay. The inter- and intra-assay analyses revealed that relative qRT-PCR is a more reliable method. Circulating miR-126 detected in the serum by relative qRT-PCRs was found low-expressed in both malignancies, significantly differentiated MM patients from healthy controls and NSCLC from MM, but do not discriminate NSCLC patients from control subjects. Kaplan-Meier analysis revealed that low level of circulating miR-126 in MM patients was strongly associated with worse prognosis. Conclusions: We propose that this approach can be adopted for accurate analysis of other suitable circulating miRNA markers of different types of cancer.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.publisherElsevier
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom575
dc.relation.ispartofpageto581
dc.relation.ispartofissue7-8
dc.relation.ispartofjournalClinical Biochemistry
dc.relation.ispartofvolume45
dc.rights.retentionY
dc.subject.fieldofresearchMedical biochemistry and metabolomics
dc.subject.fieldofresearchClinical sciences
dc.subject.fieldofresearchcode3205
dc.subject.fieldofresearchcode3202
dc.titleClinical significance of circulating miR-126 quantification in malignant mesothelioma patients
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2012
gro.hasfulltextNo Full Text
gro.griffith.authorNeuzil, Jiri


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