dc.contributor.author | Tomasetti, Marco | |
dc.contributor.author | Staffolani, Sara | |
dc.contributor.author | Nocchi, Linda | |
dc.contributor.author | Neuzil, Jiri | |
dc.contributor.author | Strafella, Elisabetta | |
dc.contributor.author | Manzella, Nicola | |
dc.contributor.author | Mariotti, Laura | |
dc.contributor.author | Bracci, Massimo | |
dc.contributor.author | Matteo, Valentino A | |
dc.contributor.author | Amati, Monica | |
dc.contributor.author | Santarelli, Lory | |
dc.date.accessioned | 2017-05-03T12:22:13Z | |
dc.date.available | 2017-05-03T12:22:13Z | |
dc.date.issued | 2012 | |
dc.date.modified | 2013-06-21T03:52:18Z | |
dc.identifier.issn | 0009-9120 | |
dc.identifier.doi | 10.1016/j.clinbiochem.2012.02.009 | |
dc.identifier.uri | http://hdl.handle.net/10072/51809 | |
dc.description.abstract | Objectives: Aim of this study was to evaluate the accuracy and precision of the detection of individual miRNA as clinical biomarkers in the serum. Design and methods: miRNA-126 was quantified in serum using endogenous and exogenous controls fornormalization and the accuracy and precision of the method evaluated. The diagnostic value of serummiRNA-126 was evaluated in malignant esothelioma (MM) and non-small-cell lung cancer (NSCLC) patients using both relative and absolute qRT-PCR methods. Results: The use of endogenous invariant and exogenous synthetic controls as well sample dilution markedly improves the accuracy and precision of the assay. The inter- and intra-assay analyses revealed that relative qRT-PCR is a more reliable method. Circulating miR-126 detected in the serum by relative qRT-PCRs was found low-expressed in both malignancies, significantly differentiated MM patients from healthy controls and NSCLC from MM, but do not discriminate NSCLC patients from control subjects. Kaplan-Meier analysis revealed that low level of circulating miR-126 in MM patients was strongly associated with worse prognosis. Conclusions: We propose that this approach can be adopted for accurate analysis of other suitable circulating miRNA markers of different types of cancer. | |
dc.description.peerreviewed | Yes | |
dc.description.publicationstatus | Yes | |
dc.language | English | |
dc.publisher | Elsevier | |
dc.publisher.place | United States | |
dc.relation.ispartofstudentpublication | N | |
dc.relation.ispartofpagefrom | 575 | |
dc.relation.ispartofpageto | 581 | |
dc.relation.ispartofissue | 7-8 | |
dc.relation.ispartofjournal | Clinical Biochemistry | |
dc.relation.ispartofvolume | 45 | |
dc.rights.retention | Y | |
dc.subject.fieldofresearch | Medical biochemistry and metabolomics | |
dc.subject.fieldofresearch | Clinical sciences | |
dc.subject.fieldofresearchcode | 3205 | |
dc.subject.fieldofresearchcode | 3202 | |
dc.title | Clinical significance of circulating miR-126 quantification in malignant mesothelioma patients | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
gro.date.issued | 2012 | |
gro.hasfulltext | No Full Text | |
gro.griffith.author | Neuzil, Jiri | |