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  • The Relationship between Fenestrations, Sieve Plates and Rafts in Liver Sinusoidal Endothelial Cells

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    Author(s)
    Svistounov, Dmitri
    Warren, Alessandra
    McNerney, Gregory P
    Owen, Dylan M
    Zencak, Dusan
    Zykova, Svetlana N
    Crane, Harry
    Huser, Thomas
    Quinn, Ronald J
    Smedsrod, Bard
    Le Couteur, David G
    Cogger, Victoria C
    Griffith University Author(s)
    Quinn, Ronald J.
    Year published
    2012
    Metadata
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    Abstract
    Fenestrations are transcellular pores in endothelial cells that facilitate transfer of substrates between blood and the extravascular compartment. In order to understand the regulation and formation of fenestrations, the relationship between membrane rafts and fenestrations was investigated in liver sinusoidal endothelial cells where fenestrations are grouped into sieve plates. Three dimensional structured illumination microscopy, scanning electron microscopy, internal reflectance fluorescence microscopy and two-photon fluorescence microscopy were used to study liver sinusoidal endothelial cells isolated from mice. There was ...
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    Fenestrations are transcellular pores in endothelial cells that facilitate transfer of substrates between blood and the extravascular compartment. In order to understand the regulation and formation of fenestrations, the relationship between membrane rafts and fenestrations was investigated in liver sinusoidal endothelial cells where fenestrations are grouped into sieve plates. Three dimensional structured illumination microscopy, scanning electron microscopy, internal reflectance fluorescence microscopy and two-photon fluorescence microscopy were used to study liver sinusoidal endothelial cells isolated from mice. There was an inverse distribution between sieve plates and membrane rafts visualized by structured illumination microscopy and the fluorescent raft stain, Bodipy FL C5 ganglioside GM1. 7-ketocholesterol and/or cytochalasin D increased both fenestrations and lipid-disordered membrane, while Triton X-100 decreased both fenestrations and lipid-disordered membrane. The effects of cytochalasin D on fenestrations were abrogated by co-administration of Triton X-100, suggesting that actin disruption increases fenestrations by its effects on membrane rafts. Vascular endothelial growth factor (VEGF) depleted lipid-ordered membrane and increased fenestrations. The results are consistent with a sieve-raft interaction, where fenestrations form in non-raft lipid-disordered regions of endothelial cells once the membrane-stabilizing effects of actin cytoskeleton and membrane rafts are diminished.
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    Journal Title
    PloS One
    Volume
    7
    Issue
    9
    DOI
    https://doi.org/10.1371/journal.pone.0046134
    Subject
    Medicinal and biomolecular chemistry not elsewhere classified
    Publication URI
    http://hdl.handle.net/10072/51858
    Collection
    • Journal articles

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