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dc.contributor.authorParizek, Martin
dc.contributor.authorDouglas, Timothy
dc.contributor.authorNovotna, Katarina
dc.contributor.authorKromka, Alexander
dc.contributor.authorBrady, Mariea
dc.contributor.authorRenzing, Andrea
dc.contributor.authorVoss, Eske
dc.contributor.authorJarosova, Marketa
dc.contributor.authorPalatinus, Lukas
dc.contributor.authorTesarek, Pavel
dc.contributor.authorRyparova, Pavla
dc.contributor.authorLisa, VĿra
dc.contributor.authordos Santos, Ana
dc.contributor.authorWarnke, Patrick
dc.contributor.authorBacakova, Lucie
dc.date.accessioned2017-05-03T16:10:20Z
dc.date.available2017-05-03T16:10:20Z
dc.date.issued2012
dc.date.modified2013-07-02T23:30:47Z
dc.identifier.issn1176-9114
dc.identifier.doi10.2147/IJN.S26665
dc.identifier.urihttp://hdl.handle.net/10072/51878
dc.description.abstractBACKGROUND: Nanofibrous scaffolds loaded with bioactive nanoparticles are promising materials for bone tissue engineering. METHODS: In this study, composite nanofibrous membranes containing a copolymer of L-lactide and glycolide (PLGA) and diamond nanoparticles were fabricated by an electrospinning technique. PLGA was dissolved in a mixture of methylene chloride and dimethyl formamide (2:3) at a concentration of 2.3 wt%, and nanodiamond (ND) powder was added at a concentration of 0.7 wt% (about 23 wt% in dry PLGA). RESULTS: In the composite scaffolds, the ND particles were either arranged like beads in the central part of the fibers or formed clusters protruding from the fibers. In the PLGA-ND membranes, the fibers were thicker (diameter 270 ᠹ nm) than in pure PLGA meshes (diameter 218 ᠴ nm), but the areas of pores among these fibers were smaller than in pure PLGA samples (0.46 ᠰ.02 孨2) versus 1.28 ᠰ.09 孨2) in pure PLGA samples). The PLGA-ND membranes showed higher mechanical resistance, as demonstrated by rupture tests of load and deflection of rupture probe at failure. Both types of membranes enabled the attachment, spreading, and subsequent proliferation of human osteoblast-like MG-63 cells to a similar extent, although these values were usually lower than on polystyrene dishes. Nevertheless, the cells on both types of membranes were polygonal or spindle-like in shape, and were distributed homogeneously on the samples. From days 1-7 after seeding, their number rose continuously, and at the end of the experiment, these cells were able to create a confluent layer. At the same time, the cell viability, evaluated by a LIVE/DEAD viability/cytotoxicity kit, ranged from 92% to 97% on both types of membranes. In addition, on PLGA-ND membranes, the cells formed well developed talin-containing focal adhesion plaques. As estimated by the determination of tumor necrosis factor-alpha levels in the culture medium and concentration of intercellular adhesion molecule-1, MG-63 cells, and RAW 264.7 macrophages on these membranes did not show considerable inflammatory activity. CONCLUSION: This study shows that nanofibrous PLGA membranes loaded with diamond nanoparticles have interesting potential for use in bone tissue engineering.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.format.extent12123213 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoeng
dc.publisherDove Medical Press
dc.publisher.placeNew Zealand
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom1931
dc.relation.ispartofpageto1951
dc.relation.ispartofjournalInternational Journal of Nanomedicine
dc.relation.ispartofvolume7
dc.rights.retentionY
dc.subject.fieldofresearchDental Materials and Equipment
dc.subject.fieldofresearchOral and Maxillofacial Surgery
dc.subject.fieldofresearchBiochemistry and Cell Biology
dc.subject.fieldofresearchNanotechnology
dc.subject.fieldofresearchPharmacology and Pharmaceutical Sciences
dc.subject.fieldofresearchcode110501
dc.subject.fieldofresearchcode110504
dc.subject.fieldofresearchcode0601
dc.subject.fieldofresearchcode1007
dc.subject.fieldofresearchcode1115
dc.titleNanofibrous poly(lactide-co-glycolide) membranes loaded with diamond nanoparticles as promising substrates for bone tissue engineering
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.rights.copyright© 2012 Martin et al, publisher and licencee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited. Please refer to the journal's website for access to the definitive, published version.
gro.date.issued2012
gro.hasfulltextFull Text
gro.griffith.authorWarnke, Patrick H.


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