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  • Longitudinal investigation of natural killer cells and cytokines in chronic fatigue syndrome/myalgic encephalomyelitis

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    Author(s)
    Brenu, Ekua W
    van Driel, Mieke L
    Staines, Donald R
    Ashton, Kevin J
    Hardcastle, Sharni L
    Keane, James
    Tajouri, Lotti
    Peterson, Daniel
    Ramos, Sandra B
    Marshall-Gradisnik, Sonya M
    Griffith University Author(s)
    Marshall-Gradisnik, Sonya M.
    Ramos, Sandra B.
    Year published
    2012
    Metadata
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    Abstract
    Background: Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME) is an etiologically unexplained disorder characterised by irregularities in various aspects of the immunological function. Presently, it is unknown whether these immunological changes remain consistent over time. This study investigates Natural Killer (NK) cell cytotoxic activity, NK cell subsets (CD56brightCD16- and CD56dimCD16+) and cytokines, over the course of a12 month period in patients with CFS/ME. Methods: The participants in the study comprised 65 (47.2 ᠱ1.5 years) CFS/ME participants and 21 (45.2 Ṯ3 years) non-fatigued controls. Flow cytometry ...
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    Background: Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME) is an etiologically unexplained disorder characterised by irregularities in various aspects of the immunological function. Presently, it is unknown whether these immunological changes remain consistent over time. This study investigates Natural Killer (NK) cell cytotoxic activity, NK cell subsets (CD56brightCD16- and CD56dimCD16+) and cytokines, over the course of a12 month period in patients with CFS/ME. Methods: The participants in the study comprised 65 (47.2 ᠱ1.5 years) CFS/ME participants and 21 (45.2 Ṯ3 years) non-fatigued controls. Flow cytometry protocols were used to assess NK subsets and NK cytotoxic activity at various time points that included baseline (T1), 6 (T2) and 12 months (T3). Cytokine secretions were measured following mitogenic stimulation of peripheral blood mononuclear cells. Results: NK cytotoxic activity was significantly decreased in the CFS/ME patients at T1, T2 and T3 compared to the non-fatigued group. Additionally, in comparison to the non-fatigued controls, the CFS/ME group had significantly lower numbers of CD56brightCD16- NK cells at both T1 and T2. Interestingly, following mitogenic stimulation, cytokine secretion revealed significant increases in IL-10, IFN-? and TNF-a at T1 in the CFS/ME group. A significant decrease was observed at T2 in the CFS/ME group for IL-10 and IL-17A while at T3, IL-2 was increased in the CFS/ME group in comparison to the non-fatigued controls. Overall cytotoxic activity was significantly decreased at T3 compared to T1 and T2. CD56brightCD16- NK cells were much lower at T2 compared to T1 and T3. IL-10 and IL-17A secretion was elevated at T2 in comparison to T1 and T3. Conclusion: These results confirm decreases in immune function in CFS/ME patients, suggesting an increased susceptibility to viral and other infections. Furthermore, NK cytotoxic activity may be a suitable biomarker for diagnosing CFS/ME as it was consistently decreased during the course of the 12 months study.
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    Journal Title
    Journal of Translational Medicine
    Volume
    10
    DOI
    https://doi.org/10.1186/1479-5876-10-88
    Copyright Statement
    © 2012 Brenu et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
    Note
    Page numbers are not for citation purposes. Instead, this article has the unique article number of 88.
    Subject
    Biomedical and clinical sciences
    Humoural immunology and immunochemistry
    Health sciences
    Publication URI
    http://hdl.handle.net/10072/51991
    Collection
    • Journal articles

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