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dc.contributor.authorE. Osborne, Sheree
dc.contributor.authorIturbe-Ormaetxe, Iñaki
dc.contributor.authorBrownlie, Jeremy
dc.contributor.authorL. O⿿Neill, Scott
dc.contributor.authorN. Johnson, Karyn
dc.date.accessioned2017-09-06T23:52:49Z
dc.date.available2017-09-06T23:52:49Z
dc.date.issued2012
dc.date.modified2014-10-09T03:40:28Z
dc.identifier.issn00992240en_US
dc.identifier.doi10.1128/AEM.01727-12en_US
dc.identifier.urihttp://hdl.handle.net/10072/52041
dc.description.abstractWolbachia, a maternally transmitted endosymbiont of insects, is increasingly being seen as an effective biological control agent that can interfere with transmission of pathogens, including dengue virus. However, the mechanism of antiviral protection is not well understood. The density and distribution of Wolbachia in host tissues have been implicated as contributing factors by previous studies with both mosquitoes and flies. Drosophila flies infected with five diverse strains of Wolbachia were screened for the ability to mediate antiviral protection. The three protective Wolbachia strains were more closely related and occurred at a higher density within whole flies than the two nonprotective Wolbachia strains. In this study, to further investigate the relationship between whole-fly Wolbachia density and the ability to mediate antiviral protection, tetracycline was used to decrease the abundance of the high-density, protective Wolbachia strain wAu prior to viral challenge. Antiviral protection was lost when the density of the protective Wolbachia strain was decreased to an abundance similar to that of nonprotective Wolbachia strains. We determined the Wolbachia density and distribution in tissues of the same five fly-Wolbachia combinations as used previously. The Wolbachia density within the head, gut, and Malpighian tubules correlated with the ability to mediate antiviral protection. These findings may facilitate the development of Wolbachia biological control strategies and help to predict host-Wolbachia pairings that may interfere with virus-induced pathology.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_US
dc.languageEnglishen_US
dc.language.isoen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.publisher.placeUnited Statesen_US
dc.relation.ispartofstudentpublicationNen_US
dc.relation.ispartofpagefrom6922en_US
dc.relation.ispartofpageto6929en_US
dc.relation.ispartofissue19en_US
dc.relation.ispartofjournalApplied and Environmental Microbiologyen_US
dc.relation.ispartofvolume78en_US
dc.rights.retentionYen_US
dc.subject.fieldofresearchcode270399en_US
dc.titleAntiviral Protection and the Importance of Wolbachia Density and Tissue Tropism in Drosophila simulansen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
dc.description.versionPublisheden_US
gro.rights.copyright© 2012 American Society for Microbiology. The attached file is reproduced here in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.en_US
gro.date.issued2012
gro.hasfulltextFull Text


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