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dc.contributor.authorPollard, Peter C
dc.date.accessioned2017-09-06T23:49:13Z
dc.date.available2017-09-06T23:49:13Z
dc.date.issued2012
dc.date.modified2013-06-28T02:08:44Z
dc.identifier.issn0099-2240
dc.identifier.doi10.1128/AEM.01268-12
dc.identifier.urihttp://hdl.handle.net/10072/52056
dc.description.abstractBulk fluorescence measurements could be a faster and cheaper way of enumerating viruses than epifluorescence microscopy, flow cytometry, or transmission electron microscopy (TEM). However, since viruses are not imaged, the background fluorescence compromises the signal, and we know little about its nature. In this paper the size ranges of nucleotides that fluoresce in the presence of SYBR gold were determined for wastewater and a range of freshwater samples using a differential filtration method. Fluorescence excitation-emission matrices (FEEMs) showed that >70% of the SYBR fluorescence was in the <10-nm size fraction (background) and was not associated with intact viruses. This was confirmed using TEM. The use of FEEMs to develop a fluorescence-based method for counting viruses is an approach that is fundamentally different from the epifluorescence microscopy technique used for enumerating viruses. This high fluorescence background is currently overlooked, yet it has had a most pervasive influence on the development of a simple fluorescence-based method for quantifying viral abundance in water.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherAmerican Society for Microbiology
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom6615
dc.relation.ispartofpageto6618
dc.relation.ispartofissue18
dc.relation.ispartofjournalApplied and Environmental Microbiology
dc.relation.ispartofvolume78
dc.rights.retentionY
dc.subject.fieldofresearchMicrobial ecology
dc.subject.fieldofresearchcode310703
dc.titleEnumerating Viruses by Using Fluorescence and the Nature of the Nonviral Background Fraction
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dc.description.versionVersion of Record (VoR)
gro.facultyGriffith Sciences, Griffith School of Environment
gro.rights.copyright© 2012 American Society for Microbiology. The attached file is reproduced here in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.
gro.date.issued2012
gro.hasfulltextFull Text
gro.griffith.authorPollard, Peter C.


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