dc.contributor.author | Huang, Yuli | |
dc.contributor.author | Nam-Trung, Nguyen | |
dc.contributor.author | Lok, Khoi Seng | |
dc.contributor.author | Lee, Peter Peng Foo | |
dc.contributor.author | Su, Maohan | |
dc.contributor.author | Wu, Min | |
dc.contributor.author | Kocgozlu, Leyla | |
dc.contributor.author | Ladoux, Benoit | |
dc.contributor.editor | Kostarelos K, Martin CR | |
dc.date.accessioned | 2017-05-03T16:14:44Z | |
dc.date.available | 2017-05-03T16:14:44Z | |
dc.date.issued | 2013 | |
dc.date.modified | 2014-04-08T04:13:36Z | |
dc.identifier.issn | 1743-5889 | |
dc.identifier.doi | 10.2217/nnm.13.45 | |
dc.identifier.uri | http://hdl.handle.net/10072/52570 | |
dc.description.abstract | Aim: This article reports the development of a multiarray microchip with real-time imaging capability to apply mechanical strains onto monolayered cell cultures. Materials & methods: Cells were cultured on an 8-孠thick membrane that was positioned in the microscope focal plane throughout the stretching process. Each stretching unit was assembled from three elastomeric layers and a glass coverslip. A programmable pneumatic control system was developed to actuate this platform. Multiple stretching experiments were conducted with various cell lines. Results: The platform provides a maximum uniform strain of 69%. Acute and long-term cell morphological changes were observed. The supreme imaging capability was verified by real-time imaging of transfected COS-7 stretching and poststretching imaging of immunofluorescence-stained PTK2. Conclusion: The platform reported here is a powerful tool for studying mechanically induced physiological changes in cells. Such a device could be used in tissue regeneration for maintaining essential cell growth conditions. | |
dc.description.peerreviewed | Yes | |
dc.description.publicationstatus | Yes | |
dc.format.extent | 1330656 bytes | |
dc.format.mimetype | application/pdf | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | Future Medicice | |
dc.publisher.place | United Kingdom | |
dc.relation.ispartofstudentpublication | Y | |
dc.relation.ispartofpagefrom | 543 | |
dc.relation.ispartofpageto | 553 | |
dc.relation.ispartofissue | 4 | |
dc.relation.ispartofjournal | Nanomedicine | |
dc.relation.ispartofvolume | 8 | |
dc.rights.retention | Y | |
dc.subject.fieldofresearch | Physical chemistry | |
dc.subject.fieldofresearch | Cell development, proliferation and death | |
dc.subject.fieldofresearch | Microelectromechanical systems (MEMS) | |
dc.subject.fieldofresearch | Medical biotechnology | |
dc.subject.fieldofresearch | Nanotechnology | |
dc.subject.fieldofresearchcode | 3406 | |
dc.subject.fieldofresearchcode | 310102 | |
dc.subject.fieldofresearchcode | 401705 | |
dc.subject.fieldofresearchcode | 3206 | |
dc.subject.fieldofresearchcode | 4018 | |
dc.title | Multiarray cell stretching platform for high magnification real-time imaging | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
gro.rights.copyright | © 2013 Future Medicine Ltd. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal website for access to the definitive, published version. | |
gro.date.issued | 2013 | |
gro.hasfulltext | Full Text | |
gro.griffith.author | Nguyen, Nam-Trung | |