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dc.contributor.authorHuang, Yuli
dc.contributor.authorNam-Trung, Nguyen
dc.contributor.authorLok, Khoi Seng
dc.contributor.authorLee, Peter Peng Foo
dc.contributor.authorSu, Maohan
dc.contributor.authorWu, Min
dc.contributor.authorKocgozlu, Leyla
dc.contributor.authorLadoux, Benoit
dc.contributor.editorKostarelos K, Martin CR
dc.date.accessioned2017-05-03T16:14:44Z
dc.date.available2017-05-03T16:14:44Z
dc.date.issued2013
dc.date.modified2014-04-08T04:13:36Z
dc.identifier.issn1743-5889
dc.identifier.doi10.2217/nnm.13.45
dc.identifier.urihttp://hdl.handle.net/10072/52570
dc.description.abstractAim: This article reports the development of a multiarray microchip with real-time imaging capability to apply mechanical strains onto monolayered cell cultures. Materials & methods: Cells were cultured on an 8-孠thick membrane that was positioned in the microscope focal plane throughout the stretching process. Each stretching unit was assembled from three elastomeric layers and a glass coverslip. A programmable pneumatic control system was developed to actuate this platform. Multiple stretching experiments were conducted with various cell lines. Results: The platform provides a maximum uniform strain of 69%. Acute and long-term cell morphological changes were observed. The supreme imaging capability was verified by real-time imaging of transfected COS-7 stretching and poststretching imaging of immunofluorescence-stained PTK2. Conclusion: The platform reported here is a powerful tool for studying mechanically induced physiological changes in cells. Such a device could be used in tissue regeneration for maintaining essential cell growth conditions.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.format.extent1330656 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoeng
dc.publisherFuture Medicice
dc.publisher.placeUnited Kingdom
dc.relation.ispartofstudentpublicationY
dc.relation.ispartofpagefrom543
dc.relation.ispartofpageto553
dc.relation.ispartofissue4
dc.relation.ispartofjournalNanomedicine
dc.relation.ispartofvolume8
dc.rights.retentionY
dc.subject.fieldofresearchPhysical chemistry
dc.subject.fieldofresearchCell development, proliferation and death
dc.subject.fieldofresearchMicroelectromechanical systems (MEMS)
dc.subject.fieldofresearchMedical biotechnology
dc.subject.fieldofresearchNanotechnology
dc.subject.fieldofresearchcode3406
dc.subject.fieldofresearchcode310102
dc.subject.fieldofresearchcode401705
dc.subject.fieldofresearchcode3206
dc.subject.fieldofresearchcode4018
dc.titleMultiarray cell stretching platform for high magnification real-time imaging
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.rights.copyright© 2013 Future Medicine Ltd. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal website for access to the definitive, published version.
gro.date.issued2013
gro.hasfulltextFull Text
gro.griffith.authorNguyen, Nam-Trung


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