MicroRNAs potentially regulate TGFb/BMP & Wnt/Ca2+ signaling pathways on modified titanium implant surfaces leading to improved osteogenicity
Author(s)
Chakravorty, N
Ivanovski, S
Prasadam, I
Crawford, R
Oloyede, A
Xiao, Y
Griffith University Author(s)
Year published
2012
Metadata
Show full item recordAbstract
Background: Sand-blasted, large grit, acid etched (SLA) and modSLA (hydrophilic SLA) titanium implant surfaces are recognized to have improved osseointegration and osteogenic properties. These properties are guided by activation of several molecular signaling pathways. This study explored the signaling pathways activated following early exposure (24 h) of osteoprogenitor cells to modSLA and SLA surfaces and their possible regulation by microRNAs. Materials and methods: Human osteoprogenitor cells were cultured on modSLA, SLA and smooth (SMO) surfaces. The relative expression of key genes involved in signaling pathways, and ...
View more >Background: Sand-blasted, large grit, acid etched (SLA) and modSLA (hydrophilic SLA) titanium implant surfaces are recognized to have improved osseointegration and osteogenic properties. These properties are guided by activation of several molecular signaling pathways. This study explored the signaling pathways activated following early exposure (24 h) of osteoprogenitor cells to modSLA and SLA surfaces and their possible regulation by microRNAs. Materials and methods: Human osteoprogenitor cells were cultured on modSLA, SLA and smooth (SMO) surfaces. The relative expression of key genes involved in signaling pathways, and miRNAs related to cell development and differentiation was evaluated. Target predictions for the differentially regulated miRNAs were performed using TargetScan and sorted for genes of TGFb/BMP and Wnt/Ca2+ pathways (for downregulated miRNAs) and inhibitors of osteogenesis (for upregulated miRNAs). Results: The study confirmed that TGFb/BMP, Wnt/Ca2+ and Notch pathways are activated within 24 h of exposure to modified surfaces. Several miRNAs showed differential expression on modSLA and SLA surfaces. Target predictions for the down-regulated miRNAs revealed several potential genes of the TGFb/BMP and Wnt/Ca2+ pathways as potential targets. Conclusion: This study demonstrated that modified titanium surfaces induce differential regulation of miRNAs that potentially regulate the TGFb/BMP andWnt/Ca2+ pathways leading to improved osteogenicity
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View more >Background: Sand-blasted, large grit, acid etched (SLA) and modSLA (hydrophilic SLA) titanium implant surfaces are recognized to have improved osseointegration and osteogenic properties. These properties are guided by activation of several molecular signaling pathways. This study explored the signaling pathways activated following early exposure (24 h) of osteoprogenitor cells to modSLA and SLA surfaces and their possible regulation by microRNAs. Materials and methods: Human osteoprogenitor cells were cultured on modSLA, SLA and smooth (SMO) surfaces. The relative expression of key genes involved in signaling pathways, and miRNAs related to cell development and differentiation was evaluated. Target predictions for the differentially regulated miRNAs were performed using TargetScan and sorted for genes of TGFb/BMP and Wnt/Ca2+ pathways (for downregulated miRNAs) and inhibitors of osteogenesis (for upregulated miRNAs). Results: The study confirmed that TGFb/BMP, Wnt/Ca2+ and Notch pathways are activated within 24 h of exposure to modified surfaces. Several miRNAs showed differential expression on modSLA and SLA surfaces. Target predictions for the down-regulated miRNAs revealed several potential genes of the TGFb/BMP and Wnt/Ca2+ pathways as potential targets. Conclusion: This study demonstrated that modified titanium surfaces induce differential regulation of miRNAs that potentially regulate the TGFb/BMP andWnt/Ca2+ pathways leading to improved osteogenicity
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Journal Title
Journal of Tissue Engineering and Regenerative Medicine
Volume
6
Issue
s1
Subject
Biomedical engineering
Clinical sciences
Periodontics
Medical physiology