Interstrand disulfide crosslinking of DNA bases supports a double nucleotide unpairing mechanism for flap endonucleases

Beddows, Amanda; Patel, Nikesh; Finger, L David; Atack, John M; Williams, David M; Grasby, Jane A

doi:10.1039/c2cc33400c

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Author(s)

Beddows, Amanda

Patel, Nikesh

Finger, L David

Atack, John M

Williams, David M

Grasby, Jane A

Griffith University Author(s)

Atack, John M.

Year published

2012

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Abstract

Flap endonucleases (FENs) are proposed to select their target phosphate diester by unpairing the two terminal nucleotides of duplex. Interstrand disulfide crosslinks, introduced by oxidation of thiouracil and thioguanine bases, abolished the specificity of human FEN1 for hydrolysis one nucleotide into the 50-duplex.Flap endonucleases (FENs) are proposed to select their target phosphate diester by unpairing the two terminal nucleotides of duplex. Interstrand disulfide crosslinks, introduced by oxidation of thiouracil and thioguanine bases, abolished the specificity of human FEN1 for hydrolysis one nucleotide into the 50-duplex.
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Journal Title

ChemComm

Volume

DOI

https://doi.org/10.1039/c2cc33400c

Copyright Statement

© 2012 Royal Society of Chemistry. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal website for access to the definitive, published version.

Subject

Chemical sciences

Enzymes

Publication URI

http://hdl.handle.net/10072/52995

Collection

Journal articles