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dc.contributor.authorLok, Khoi Seng
dc.contributor.authorKwok, Yien Chian
dc.contributor.authorNguyen, Nam-Trung
dc.date.accessioned2017-05-03T16:14:39Z
dc.date.available2017-05-03T16:14:39Z
dc.date.issued2012
dc.date.modified2014-08-11T00:46:14Z
dc.identifier.issn0026-3672
dc.identifier.doi10.1007/s00604-011-0741-x
dc.identifier.urihttp://hdl.handle.net/10072/53118
dc.description.abstractWe report on a novel concept of sample loading for microfluidic devices using a benchtop centrifuge and a magnetically actuated circular closed-loop PCR microchip as a model system. The PCR mixture and the ferrofluid were loaded into a specially designed microchip. The microchip was then placed in an off-the-shelf 50-mL tube and centrifuged. The strong centrifugal force drives the PCR mixture and the ferrofluid into the microchannels of the microchip, and simultaneously expels any trapped microbubbles. PCR was successfully carried out on single and parallel closed-loop PCR microchips. The addition of a few off-chip handling steps allows great simplification of the device design. This new loading concept may be useful for designing robust and low-cost lab-on-a-chip devices because benchtop centrifuges are quite common in most laboratories.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.format.extent440608 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoeng
dc.publisherSpringer
dc.publisher.placeAustria
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom445
dc.relation.ispartofpageto453
dc.relation.ispartofissue3-4
dc.relation.ispartofjournalMicrochimica Acta
dc.relation.ispartofvolume176
dc.rights.retentionY
dc.subject.fieldofresearchAnalytical chemistry
dc.subject.fieldofresearchInstrumental methods (excl. immunological and bioassay methods)
dc.subject.fieldofresearchOther chemical sciences
dc.subject.fieldofresearchMicroelectromechanical systems (MEMS)
dc.subject.fieldofresearchcode3401
dc.subject.fieldofresearchcode340105
dc.subject.fieldofresearchcode3499
dc.subject.fieldofresearchcode401705
dc.titleSample loading and retrieval by centrifugation in a closed-loop PCR microchip
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.rights.copyright© 2012 Springer Vienna. This is an electronic version of an article published in Microchimica Acta, Vol. 176(3-4), pp. 445-453, 2012. Microchimica Acta is available online at: http://link.springer.com/ with the open URL of your article.
gro.date.issued2012
gro.hasfulltextFull Text
gro.griffith.authorNguyen, Nam-Trung


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