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dc.contributor.authorParks, M
dc.contributor.authorListon, A
dc.contributor.authorCronn, R
dc.date.accessioned2017-05-03T16:08:01Z
dc.date.available2017-05-03T16:08:01Z
dc.date.issued2011
dc.date.modified2013-09-17T23:08:28Z
dc.identifier.issn0002-9122
dc.identifier.doi10.3732/ajb.1100088
dc.identifier.urihttp://hdl.handle.net/10072/53172
dc.description.abstract堐remise of the study: Primers were designed to amplify the highly variable locus ycf1 from all 11 subsections of Pinus to facilitate plastome assemblies based on short sequence reads as well as future phylogenetic and population genetic analyses. 堍ethods and Results: Primer design was based on alignment of 33 Pinus and four Pinaceae plastomes with mostly incomplete ycf1 sequences. Sanger sequencing of 12 Pinus accessions resulted in open reading frames ranging in size from 5.2 to 6.1 kbp. Highest sequence diversity was identified in two regions totaling 5.5 kbp aligned length, which can be targeted in all pine subsections with three primer combinations. Preliminary results suggest the primers described also amplify homologous targets in the broader Pinaceae. 堃onclusions: The successful design and implementation of PCR primers spanning the large, variable locus ycf1 in Pinus represents the development of a valuable tool in pine genetic studies, and should facilitate studies throughout Pinaceae.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherBotanical Society of America
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrome185
dc.relation.ispartofpagetoe188
dc.relation.ispartofissue7
dc.relation.ispartofjournalAmerican Journal of Botany
dc.relation.ispartofvolume98
dc.rights.retentionY
dc.subject.fieldofresearchPlant Systematics and Taxonomy
dc.subject.fieldofresearchTechnology not elsewhere classified
dc.subject.fieldofresearchEcology
dc.subject.fieldofresearchEvolutionary Biology
dc.subject.fieldofresearchPlant Biology
dc.subject.fieldofresearchcode060310
dc.subject.fieldofresearchcode109999
dc.subject.fieldofresearchcode0602
dc.subject.fieldofresearchcode0603
dc.subject.fieldofresearchcode0607
dc.titleNewly developed primers for complete ycf1 amplification in Pinus (Pinaceae) chloroplasts with possible family-wide utility
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2011
gro.hasfulltextNo Full Text
gro.griffith.authorParks, Matthew


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