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dc.contributor.authorDando, Samantha J.
dc.contributor.authorNitsos, Ilias
dc.contributor.authorKallapur, Suhas G.
dc.contributor.authorNewnham, John
dc.contributor.authorPolglase, Graeme R.
dc.contributor.authorJane Pillow, J.
dc.contributor.authorJobe, Alan H.
dc.contributor.authorTimms, Peter
dc.contributor.authorKnox, Christine L.
dc.date.accessioned2017-05-03T16:10:51Z
dc.date.available2017-05-03T16:10:51Z
dc.date.issued2012
dc.date.modified2013-10-16T22:16:50Z
dc.identifier.issn19326203
dc.identifier.doi10.1371/journal.pone.0029856
dc.identifier.urihttp://hdl.handle.net/10072/53175
dc.description.abstractThe multiple banded antigen (MBA) is a predicted virulence factor of Ureaplasma species. Antigenic variation of the MBA is a potential mechanism by which ureaplasmas avoid immune recognition and cause chronic infections of the upper genital tract of pregnant women. We tested whether the MBA is involved in the pathogenesis of intra-amniotic infection and chorioamnionitis by injecting virulent or avirulent-derived ureaplasma clones (expressing single MBA variants) into the amniotic fluid of pregnant sheep. At 55 days of gestation pregnant ewes (n = 20) received intra-amniotic injections of virulent-derived or avirulent-derived U. parvum serovar 6 strains (2ױ04 CFU), or 10B medium (n = 5). Amniotic fluid was collected every two weeks post-infection and fetal tissues were collected at the time of surgical delivery of the fetus (140 days of gestation). Whilst chronic colonisation was established in the amniotic fluid of animals infected with avirulent-derived and virulent-derived ureaplasmas, the severity of chorioamnionitis and fetal inflammation was not different between these groups (p>0.05). MBA size variants (32-170 kDa) were generated in vivo in amniotic fluid samples from both the avirulent and virulent groups, whereas in vitro antibody selection experiments led to the emergence of MBA-negative escape variants in both strains. Anti-ureaplasma IgG antibodies were detected in the maternal serum of animals from the avirulent (40%) and virulent (55%) groups, and these antibodies correlated with increased IL-1߬ IL-6 and IL-8 expression in chorioamnion tissue (p<0.05). We demonstrate that ureaplasmas are capable of MBA phase variation in vitro; however, ureaplasmas undergo MBA size variation in vivo, to potentially prevent eradication by the immune response. Size variation of the MBA did not correlate with the severity of chorioamnionitis. Nonetheless, the correlation between a maternal humoral response and the expression of chorioamnion cytokines is a novel finding. This host response may be important in the pathogenesis of inflammation-mediated adverse pregnancy outcomes.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.format.extent1180660 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoeng
dc.publisherPLOS
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrome29856 - 1
dc.relation.ispartofpagetoe29856 - 15
dc.relation.ispartofissue1
dc.relation.ispartofjournalPLoS One
dc.relation.ispartofvolume7
dc.rights.retentionY
dc.subject.fieldofresearchMedical Bacteriology
dc.subject.fieldofresearchcode110801
dc.titleThe Role of the Multiple Banded Antigen of Ureaplasma parvum in Intra-Amniotic Infection: Major Virulence Factor or Decoy?
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://www.plos.org/journals/license.html
gro.rights.copyright© 2012 Dando et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License CCAL. (http://www.plos.org/journals/license.html)
gro.date.issued2012
gro.hasfulltextFull Text
gro.griffith.authorDando, Samantha


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