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dc.contributor.authorP. Busch, Michael
dc.contributor.authorK. Murthy, Krishna
dc.contributor.authorH. Kleinman, Steven
dc.contributor.authorF. Hirschkorn, Dale
dc.contributor.authorL. Herring, Belinda
dc.contributor.authorL. Delwart, Eric
dc.contributor.authorRacanelli, Vito
dc.contributor.authorYoon, Joo Chun
dc.contributor.authorRehermann, Barbara
dc.contributor.authorJ. Alter, Harvey
dc.date.accessioned2017-05-03T16:10:43Z
dc.date.available2017-05-03T16:10:43Z
dc.date.issued2012
dc.date.modified2013-09-19T23:29:23Z
dc.identifier.issn15280020
dc.identifier.doi10.1182/blood-2011-12-393637
dc.identifier.urihttp://hdl.handle.net/10072/53267
dc.description.abstractSerial plasma aliquots (50 mL) obtained from 10 commercial donors who converted from hepatitis C virus (HCV) RNA negative to positive were transfused into 2 chimpanzees to assess infectivity during early HCV infection. Plasma, obtained 4 days before HCV RNA detectability by licensed assays, transmitted HCV infection to chimpanzee X355. The infectious PCR-negative plasma was subsequently shown to be positive in 2 of 23 replicates using a sensitive transcription-mediated amplification (TMA) assay, and estimated to contain 1.2 HCV RNA copies/mL (60 copies/50 mL transfused). Plasma units obtained up to 8 weeks earlier were not infectious in a second susceptible chimp, even when from donors with low-level, intermittent HCV RNA detection. Chimp x355 developed acute viremia with subsequent seroconversion, but cleared both virus and Ab in 17 weeks. When rechallenged 38 months later with 6000 RNA copies/mL from the same donor, X355 was transiently reinfected and again rapidly lost all HCV markers. We conclude that: (1) transfusions can transmit HCV infection before RNA detection, but the interval of test-negative infectivity is very brief; (2) early "blips" of HCV RNA appear noninfectious and can be ignored when calculating residual transfusion risk; and (3) markers of HCV infection can be lost rapidly after exposure to low-dose inocula.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherAmerican Society for Haematology
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom6326
dc.relation.ispartofpageto6334
dc.relation.ispartofissue26
dc.relation.ispartofjournalBlood
dc.relation.ispartofvolume119
dc.rights.retentionY
dc.subject.fieldofresearchVirology
dc.subject.fieldofresearchCardiorespiratory Medicine and Haematology
dc.subject.fieldofresearchClinical Sciences
dc.subject.fieldofresearchPaediatrics and Reproductive Medicine
dc.subject.fieldofresearchcode060506
dc.subject.fieldofresearchcode1102
dc.subject.fieldofresearchcode1103
dc.subject.fieldofresearchcode1114
dc.titleInfectivity in chimpanzees (Pan troglodytes) of plasma collected before HCV RNAdetectability by FDA-licensed assays: implications for transfusion safety and HCV infection outcomes
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.rights.copyrightSelf-archiving of the author-manuscript version is not yet supported by this journal. Please refer to the journal link for access to the definitive, published version or contact the author[s] for more information.
gro.date.issued2012
gro.hasfulltextNo Full Text
gro.griffith.authorHerring, Belinda


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