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  • Real-Time Monitoring of Ca2+ Concentration in Pancreatic Beta Cells by a Microfluidic Device Integrated with Total Internal Reflection (TIR)-Based Chip

    Author(s)
    Kitazawa, Yuko
    Yokokawa, Ryuji
    Terao, Kyohei
    Okonogi, Atsuhito
    Dao, Dzung Viet
    Sugiyama, Susumu
    Kanno, Isaku
    Kotera, Hidetoshi
    Griffith University Author(s)
    Dao, Dzung V.
    Year published
    2011
    Metadata
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    Abstract
    We present a microfluidic device integrated with a Total Internal Reflection (TIR)-based chip for cell observation and analysis, which enables Total Internal Reflection Fluorescence Microscopy (TIRFM). By integrating the microfluidic device with optical components, the assembly becomes unnecessary. An evanescent field generated by the TIR-based chip was demonstrated by observation of fluorescent beads and insulin granules in mouse pancreatic beta cells, MIN6-m9. Comparing with epi-fluorescence microscopy (EPIFM), high S/N ratio of 4-fold was realized by the TIR-based chip. To demonstrate the utility of the device integration, ...
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    We present a microfluidic device integrated with a Total Internal Reflection (TIR)-based chip for cell observation and analysis, which enables Total Internal Reflection Fluorescence Microscopy (TIRFM). By integrating the microfluidic device with optical components, the assembly becomes unnecessary. An evanescent field generated by the TIR-based chip was demonstrated by observation of fluorescent beads and insulin granules in mouse pancreatic beta cells, MIN6-m9. Comparing with epi-fluorescence microscopy (EPIFM), high S/N ratio of 4-fold was realized by the TIR-based chip. To demonstrate the utility of the device integration, we analyzed intracellular Ca2+ concentration change in MIN6-m9 cells in response to KCl stimulation. We found out our integrated device realized quick perfusion for cell analysis with keeping high S/N ratio
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    Conference Title
    2011 IEEE 24TH INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS)
    Publisher URI
    http://www.ieee-mems2011.org/
    DOI
    https://doi.org/10.1109/MEMSYS.2011.5734585
    Subject
    Biomedical instrumentation
    Publication URI
    http://hdl.handle.net/10072/53346
    Collection
    • Conference outputs

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