Combining Protein Fragment Feature-Based Resampling and Local Optimisation
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Protein structure prediction (PSP) suites can predict 'nearnative' protein models. However, not always these predicted models are close to the native structure with enough precision to be useful for biologists. The literature to date demonstrates that one of the best techniques to predict 'near-native' protein models is to use a fragment-based search strategy. Another technique that can help refine protein models is local optimisation. Local optimisation algorithms use the gradient of the function being optimised to suggest which move will bring the function value closer to its local minimum. In this work we combine the concepts of structural refinement through feature-based resampling, fragment-based PSP, and local optimisation to create an algorithm that can create protein models that are closer to their native states. In experiments we demonstrated that our new method generates models that are close to their native conformations. For structures in the test set, it obtained an average RMSD of 5.09`and an average best TM-Score of 0.47 when no local optimisation was applied. However, by applying local optimisation to our algorithm, additional improvements were achieved.
Eighth IAPR International Conference on Pattern Recognition in Bioinformatics, Lecture Notes in Computer Science, Vol. 7986
Copyright 2013 Springer-Verlag Berlin Heidelberg. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the conference's website for access to the definitive, published version.
Pattern Recognition and Data Mining