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dc.contributor.authorGradin, Per
dc.contributor.authorHollberg, Karin
dc.contributor.authorCassady, A Ian
dc.contributor.authorLang, Pernilla
dc.contributor.authorAndersson, Goran
dc.date.accessioned2017-05-03T16:15:30Z
dc.date.available2017-05-03T16:15:30Z
dc.date.issued2012
dc.date.modified2013-10-30T04:08:37Z
dc.identifier.issn1422-6405
dc.identifier.doi10.1159/000330806
dc.identifier.urihttp://hdl.handle.net/10072/53945
dc.description.abstractBone remodeling is a central event in the maintenance of skeletal tissue, and involves cycles of resorption followed by the formation of bone tissue. The activity of osteoclasts and osteoblasts during these cycles is tightly regulated by sys- temic and local factors coupling the action of these cells. Tar- trate-resistant acid phosphatase (TRAP) is predominantly ex- pressed in bone by osteoclasts but has also been detected in osteoblasts and osteocytes. Moreover, TRAP can stimulate the differentiation of mesenchymal lineage cells, i.e. progen- itors of osteoblasts and adipocytes. In order to further ex- plore the effects of TRAP on bone turnover, the structural and molecular phenotypes of osteoclasts and osteoblasts were assessed in TRAP-overexpressing transgenic mice. Transgenic mice of both sexes display increased cortical bone mineral content and density, which cannot be account- ed for by decreased bone resorption since osteoclast num- bers and resorptive activity do not differ from wild-type mice. Examination of the osteoblast phenotype revealed that markers of bone formation, i.e. procollagen type I N- terminal propeptides, and osteoblast lineage markers as well as the TRAP 1B mRNA transcript are increased in TRAP- overexpressing mice. Expression of the osteoclast-selective TRAP 1C mRNA is not increased in TRAP transgenic mice. El- evated expression of TRAP mRNA and protein were detected in osteoblasts, osteocytes and in the bone matrix of TRAP transgenic mice, suggesting that TRAP overexpression in os- teoblast lineage cells is associated with increased cortical bone mineral content and density. The data presented here support the hypothesis that TRAP overexpression in the os- teoblastic cell lineage stimulates the differentiation and/or activation of these cells.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherS. Karger AG
dc.publisher.placeSwitzerland
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom68
dc.relation.ispartofpageto81
dc.relation.ispartofissue1
dc.relation.ispartofjournalCells, Tissues, Organs
dc.relation.ispartofvolume196
dc.rights.retentionY
dc.subject.fieldofresearchBiochemistry and cell biology
dc.subject.fieldofresearchAnimal cell and molecular biology
dc.subject.fieldofresearchOrthopaedics
dc.subject.fieldofresearchMedical physiology
dc.subject.fieldofresearchcode3101
dc.subject.fieldofresearchcode310902
dc.subject.fieldofresearchcode320216
dc.subject.fieldofresearchcode3208
dc.titleTransgenic Overexpression of Tartrate-Resistant Acid Phosphatase Is Associated with Induction of Osteoblast Gene Expression and Increased Cortical Bone Mineral Content and Density
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2012
gro.hasfulltextNo Full Text
gro.griffith.authorCassady, Ian


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