miR1288 in colorectal cancers: Altered expression and its clinicopathological significance

Abstract

Introduction: MicroRNA-1288 (miR-1288) is a non-coding RNA located in 17p11.2 and not been studied solely in cancer. We aim to examine the miR-1288 expression in a large cohort of patients with colorectal carcinoma, adenoma and non-neoplastic colorectal tissues. The implications of miR-1288 expression and clinicopathological features were also studied. Methods: Tissues from 102 patients with surgical resection of colorectum (82 adenocarcinomas, 10 adenomas and 10 non-neoplastic tissues), two colon cancer cell lines (SW480 and SW48) and one normal colonic epithelial cell line (FHC) were recruited. miRNA was extracted from all these samples and were converted in to cDNA. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed for the detection of miR-1288 expression. The results were correlated with the clinical and pathological data. Results: miR-1288 expression was decreased in majority of colorectal adenocarcinoma when compared to colorectal adenoma and non-neoplastic tissues. Reduced or absence expression of miR-1288 was noted in 78% (n=64) of the cancers. The colon cancer cell lines showed reduced expression of miR-1288 compared to normal colonic epithelial cell line. Distal colorectal cancers showed higher expression levels of miR-1288 than proximal cancers (p= 0.007). The expression levels of miR-1288 were significantly altered in different levels of cancer invasion (T staging) (p= 0.023). Conclusions: Altered expression pattern of miR-1288 in colorectal cancers compare to non-neoplastic tissues was identified. Also, differential regulation of miR-1288 was found to be related to location and pathological staging of colorectal cancers.