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dc.contributor.authorMoelzer, Christine
dc.contributor.authorPfleger, Barbara
dc.contributor.authorPutz, Elisabeth
dc.contributor.authorRossmann, Antonia
dc.contributor.authorSchwarz, Ursula
dc.contributor.authorWallner, Marlies
dc.contributor.authorBulmer, Andrew C
dc.contributor.authorWagner, Karl-Heinz
dc.date.accessioned2017-05-03T15:40:16Z
dc.date.available2017-05-03T15:40:16Z
dc.date.issued2013
dc.date.modified2013-11-11T22:23:43Z
dc.identifier.issn0014-4827
dc.identifier.doi10.1016/j.yexcr.2012.12.003
dc.identifier.urihttp://hdl.handle.net/10072/54168
dc.description.abstractEpidemiological studies report a negative association between circulating bilirubin concentrations and the risk for cancer and cardiovascular disease. Structurally related tetrapyrroles also possess in vitro anti-genotoxic activity and may prevent mutation prior to malignancy. Furthermore, few data suggest that tetrapyrroles exert anti-carcinogenic effects via induction of cell cycle arrest and apoptosis. To further investigate whether tetrapyrroles provoke DNA-damage in human cancer cells, they were tested in the single cell gel electrophoresis assay (SCGE). Eight tetrapyrroles (unconjugated bilirubin, bilirubin ditaurate, biliverdin, biliverdin-/bilirubin dimethyl ester, urobilin, stercobilin and protoporphyrin) were added to cultured Caco2 and HepG2 cells and their effects on comet formation (% tail DNA) were assessed. Flow cytometric assessment (apoptosis/necrosis, cell cycle, intracellular radical species generation) assisted in revealing underlying mechanisms of intracellular action. Cells were incubated with tetrapyrroles at concentrations of 0.5, 5 and 17 占for 24 h. Addition of 300 占tertiary-butyl hydroperoxide to cells served as a positive control. Tetrapyrrole incubation mostly resulted in increased DNA-damage (comet formation) in Caco2 and HepG2 cells. Tetrapyrroles that are concentrated within the intestine, including protoporphyrin, urobilin and stercobilin, led to significant comet formation in both cell lines, implicating the compounds in inducing DNA-damage and apoptosis in cancer cells found within organs of the digestive system.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom536
dc.relation.ispartofpageto545
dc.relation.ispartofissue4
dc.relation.ispartofjournalExperimental Cell Research
dc.relation.ispartofvolume319
dc.rights.retentionY
dc.subject.fieldofresearchCancer Cell Biology
dc.subject.fieldofresearchBiochemistry and Cell Biology
dc.subject.fieldofresearchClinical Sciences
dc.subject.fieldofresearchcode111201
dc.subject.fieldofresearchcode0601
dc.subject.fieldofresearchcode1103
dc.titleIn vitro DNA-damaging effects of intestinal and related tetrapyrroles in human cancer cells
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2013
gro.hasfulltextNo Full Text
gro.griffith.authorBulmer, Andrew C.
gro.griffith.authorWagner, Karl-Heinz


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