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dc.contributor.authorHatzirodos, Nicholas
dc.contributor.authorNigro, Julie
dc.contributor.authorIrving-Rodgers, Helen F
dc.contributor.authorVashi, Aditya V
dc.contributor.authorHummitzsch, Katja
dc.contributor.authorCaterson, Bruce
dc.contributor.authorSullivan, Thomas R
dc.contributor.authorRodgers, Raymond J
dc.date.accessioned2017-05-03T16:13:10Z
dc.date.available2017-05-03T16:13:10Z
dc.date.issued2012
dc.date.modified2013-11-11T22:49:48Z
dc.identifier.issn0945-053X
dc.identifier.doi10.1016/j.matbio.2011.10.002
dc.identifier.urihttp://hdl.handle.net/10072/54236
dc.description.abstractTo examine the detailed composition of glycosaminoglycans during bovine ovarian follicular development and atresia, the specialized stromal theca layers were separated from the stratified epithelial granulosa cells of healthy (n = 6) and atretic (n = 6) follicles in each of three size ranges: small (3–5 mm), medium (6-9 mm) and large (10 mm or more) (n = 29 animals). Fluorophore-assisted carbohydrate electrophoresis analyses (on a per cell basis) and immunohistochemistry (n = 14) were undertaken. We identified the major disaccharides in thecal layers and the membrana granulosa as chondroitin sulfate-derived ∆uronic acid with 4-sulfated N-acetylgalactosamine and ∆uronic acid with 6-sulfated N-acetylgalactosamine and the heparan sulfate-derived Δuronic acid with N-acetlyglucosamine, with elevated levels in the thecal layers. Increasing follicle size and atresia was associated with increased levels of some disaccharides. We concluded that versican contains 4-sulfated N-acetylgalactosamine and it is the predominant 4-sulfated N-acetylgalactosamine proteoglycan in antral follicles. At least one other non- or 6-sulfated N-acetylgalactosamine proteoglycan(s), which is not decorin or an inter-α-trypsin inhibitor family member, is present in bovine antral follicles and associated with hitherto unknown groups of cells around some larger blood vessels. These areas stained positively for chondroitin/dermatan sulfate epitopes [antibodies 7D4, 3C5, and 4C3], similar to stem cell niches observed in other tissues. The sulfation pattern of heparan sulfate glycosaminoglycans appears uniform across follicles of different sizes and in healthy and atretic follicles. The heparan sulfate products detected in the follicles are likely to be associated with perlecan, collagen XVIII or betaglycan.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.publisher.placeNetherlands
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom45
dc.relation.ispartofpageto56
dc.relation.ispartofissue1
dc.relation.ispartofjournalMatrix Biology
dc.relation.ispartofvolume31
dc.rights.retentionY
dc.subject.fieldofresearchBiological sciences
dc.subject.fieldofresearchAnimal developmental and reproductive biology
dc.subject.fieldofresearchcode31
dc.subject.fieldofresearchcode310903
dc.titleGlycomic analyses of ovarian follicles during development and atresia
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2012
gro.hasfulltextNo Full Text
gro.griffith.authorIrving-Rodgers, Helen F.


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