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  • Dynamics of extracellular matrix in ovarian follicles and corpora lutea of mice

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    Author(s)
    Irving-Rodgers, Helen F
    Hummitzsch, Katja
    Murdiyarso, Lydia S
    Bonner, Wendy M
    Sado, Yoshikazu
    Ninomiya, Yoshifumi
    Couchman, John R
    Sorokin, Lydia M
    Rodgers, Raymond J
    Griffith University Author(s)
    Irving-Rodgers, Helen F.
    Year published
    2010
    Metadata
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    Abstract
    Despite the mouse being an important laboratory species, little is known about changes in its extracellular matrix (ECM) during follicle and corpora lutea formation and regression. Follicle development was induced in mice (29 days of age/experimental day 0) by injections of pregnant mare’s serum gonadotrophin on days 0 and 1 and ovulation was induced by injection of human chorionic gonadotrophin on day 2. Ovaries were collected for immunohistochemistry (n=10 per group) on days 0, 2 and 5. Another group was mated and ovaries were examined on day 11 (n=7). Collagen type IV α1 and α2, laminin α1, β1 and γ1 chains, nidogens 1 ...
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    Despite the mouse being an important laboratory species, little is known about changes in its extracellular matrix (ECM) during follicle and corpora lutea formation and regression. Follicle development was induced in mice (29 days of age/experimental day 0) by injections of pregnant mare’s serum gonadotrophin on days 0 and 1 and ovulation was induced by injection of human chorionic gonadotrophin on day 2. Ovaries were collected for immunohistochemistry (n=10 per group) on days 0, 2 and 5. Another group was mated and ovaries were examined on day 11 (n=7). Collagen type IV α1 and α2, laminin α1, β1 and γ1 chains, nidogens 1 and 2 and perlecan were present in the follicular basal lamina of all developmental stages. Collagen type XVIII was only found in basal lamina of primordial, primary and some preantral follicles, whereas laminin α2 was only detected in some preantral and antral follicles. The focimatrix, a specialised matrix of the membrana granulosa, contained collagen type IV α1 and α2, laminin α1, β1 and γ1 chains, nidogens 1 and 2, perlecan and collagen type XVIII. In the corpora lutea, staining was restricted to capillary sub-endothelial basal laminas containing collagen type IV α1 and α2, laminin α1, β1 and γ1 chains, nidogens 1 and 2, perlecan and collagen type XVIII. Laminins α4 and α5 were not immunolocalised to any structure in the mouse ovary. The ECM composition of the mouse ovary has similarities to, but also major differences from, other species with respect to nidogens 1 and 2 and perlecan.
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    Journal Title
    Cell and Tissue Research
    Volume
    339
    Issue
    3
    DOI
    https://doi.org/10.1007/s00441-009-0905-8
    Copyright Statement
    © The Author(s) 2010. This is a Springer Open Choice license agreement which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
    Subject
    Medical physiology
    Systems physiology
    Publication URI
    http://hdl.handle.net/10072/54237
    Collection
    • Journal articles

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