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dc.contributor.authorMcCaskill, Jana L
dc.contributor.authorMarsh, Glenn A
dc.contributor.authorMonaghan, Paul
dc.contributor.authorWang, Lin-Fa
dc.contributor.authorDoran, Timothy
dc.contributor.authorMcMillan, Nigel AJ
dc.date.accessioned2017-08-31T12:30:52Z
dc.date.available2017-08-31T12:30:52Z
dc.date.issued2013
dc.date.modified2013-12-03T00:13:24Z
dc.identifier.issn1932-6203
dc.identifier.doi10.1371/journal.pone.0064360
dc.identifier.urihttp://hdl.handle.net/10072/54750
dc.description.abstractHendra virus (HeV) is a highly pathogenic zoonotic paramyxovirus that causes fatal disease in a wide range of species, including humans. HeV was first described in Australia in 1994, and has continued to re-emerge with increasing frequency. HeV is of significant concern to human health due to its high mortality rate, increasing emergence, absence of vaccines and limited post exposure therapies. Here we investigate the use of RNA interference (RNAi) based therapeutics targeting HeV in conjunction with the TLR3 agonist Poly I:C and show that they are potent inhibitors of HeV infection in vitro. We found that short interfering RNAs (siRNAs) targeting the abundantly expressed N, P and M genes of HeV caused over 95% reduction of HeV virus titre, protein and mRNA. Furthermore, we found that the combination of HeV targeting siRNA and Poly I:C had an additive effect in suppressing HeV infection. Our results demonstrate for the first time that RNAi and type I interferon stimulation are effective inhibitors of HeV replication in vitro and may provide an effective therapy for this highly lethal, zoonotic pathogen.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.format.extent877560 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.publisherPublic Library of Sciences
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationY
dc.relation.ispartofpagefrome64360-1
dc.relation.ispartofpagetoe64360-9
dc.relation.ispartofissue5
dc.relation.ispartofjournalPLoS One
dc.relation.ispartofvolume8
dc.rights.retentionY
dc.subject.fieldofresearchGene and Molecular Therapy
dc.subject.fieldofresearchcode100401
dc.titlePotent Inhibition of Hendra Virus Infection via RNA Interference and Poly I:C Immune Activation
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://www.plos.org/journals/license.html
gro.rights.copyright© 2013 McCaskill et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License CCAL. (http://www.plos.org/journals/license.html)
gro.date.issued2013
gro.hasfulltextFull Text
gro.griffith.authorMcMillan, Nigel


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