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dc.contributor.authorStuckey, Daniel J.
dc.contributor.authorCarr, Carolyn A.
dc.contributor.authorMartin-Rendon, Enca
dc.contributor.authorTyler, Damian J.
dc.contributor.authorWillmott, Corinne
dc.contributor.authorCassidy, Paul J.
dc.contributor.authorHale, Sarah J.M.
dc.contributor.authorSchneider, Jürgen E.
dc.contributor.authorTatton, Louise
dc.contributor.authorHarding, Sian E.
dc.contributor.authorRadda, George K.
dc.contributor.authorWatt, Suzanne
dc.contributor.authorClarke, Kiernan
dc.date.accessioned2017-05-03T15:55:15Z
dc.date.available2017-05-03T15:55:15Z
dc.date.issued2006
dc.date.modified2013-12-12T02:55:56Z
dc.identifier.issn1066-5099
dc.identifier.doi10.1634/stemcells.2006-0074
dc.identifier.urihttp://hdl.handle.net/10072/54851
dc.description.abstractStem cells offer a promising approach to the treatment of myocardial infarction and prevention of heart failure. We have used iron labeling of bone marrow stromal cells (BMSCs) to noninvasively track cell location in the infarcted rat heart over 16 weeks using cine-magnetic resonance imaging (cine-MRI) and to isolate the BMSCs from the grafted hearts using the magnetic properties of the donor cells. BMSCs were isolated from rat bone marrow, characterized by flow cytometry, transduced with lentiviral vectors expressing green fluorescent protein (GFP), and labeled with iron particles. BMSCs were injected into the infarct periphery immediately following coronary artery ligation, and rat hearts were imaged at 1, 4, 10, and 16 weeks postinfarction. Signal voids caused by the iron particles in the BMSCs were detected in all rats at all time points. In mildly infarcted hearts, the volume of the signal void decreased over the 16 weeks, whereas the signal void volume did not decrease significantly in severely infarcted hearts. High-resolution three-dimensional magnetic resonance (MR) microscopy identified hypointense regions at the same position as in vivo. Donor cells containing iron particles and expressing GFP were identified in MR-targeted heart sections after magnetic cell separation from digested hearts. In conclusion, MRI can be used to track cells labeled with iron particles in damaged tissue for at least 16 weeks after injection and to guide tissue sectioning by accurately identifying regions of cell engraftment. The magnetic properties of the iron-labeled donor cells can be used for their isolation from host tissue to enable further characterization.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherAlphaMed Press, Inc.
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom1968
dc.relation.ispartofpageto1975
dc.relation.ispartofissue8
dc.relation.ispartofjournalStem Cells
dc.relation.ispartofvolume24
dc.rights.retentionY
dc.subject.fieldofresearchMedical Physics
dc.subject.fieldofresearchBiological Sciences
dc.subject.fieldofresearchTechnology
dc.subject.fieldofresearchMedical and Health Sciences
dc.subject.fieldofresearchcode029903
dc.subject.fieldofresearchcode06
dc.subject.fieldofresearchcode10
dc.subject.fieldofresearchcode11
dc.titleIron Particles for Noninvasive Monitoring of Bone Marrow Stromal Cell Engraftment into, and Isolation of Viable Engrafted Donor Cells from, the Heart
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.rights.copyrightSelf-archiving of the author-manuscript version is not yet supported by this journal. Please refer to the journal link for access to the definitive, published version or contact the author[s] for more information.
gro.date.issued2006
gro.hasfulltextNo Full Text
gro.griffith.authorCassidy, Paul


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