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dc.contributor.authorWatanabe, L
dc.contributor.authorNirthanan, S
dc.contributor.authorRajaseger, G
dc.contributor.authorPolikarpov, I
dc.contributor.authorKini, RM
dc.contributor.authorArni, RK
dc.date.accessioned2009-07-09
dc.date.accessioned2013-12-17T21:52:19Z
dc.date.accessioned2017-03-02T01:05:19Z
dc.date.available2017-03-02T01:05:19Z
dc.date.issued2002
dc.date.modified2013-12-17T21:52:19Z
dc.identifier.issn0907-4449
dc.identifier.doi10.1107/S0907444902011022
dc.identifier.urihttp://hdl.handle.net/10072/55049
dc.description.abstractBucain is a three-finger toxin, structurally homologous to snake-venom muscarinic toxins, from the venom of the Malayan krait Bungarus candidus. These proteins have molecular masses of approximately 6000-8000 Da and encompass the potent curaremimetic neurotoxins which confer lethality to Elapidae and Hydrophidae venoms. Bucain was crystallized in two crystal forms by the hanging-drop vapour-diffusion technique in 0.1 M sodium citrate pH 5.6, 15% PEG 4000 and 0.15 M ammonium acetate. Form I crystals belong to the monoclinic system space group C2, with unit-cell parameters a = 93.73, b = 49.02, c = 74.09 A, beta = 111.32 degrees, and diffract to a nominal resolution of 1.61 A. Form II crystals also belong to the space group C2, with unit-cell parameters a = 165.04, b = 49.44, c = 127.60 A, beta = 125.55 degrees, and diffract to a nominal resolution of 2.78 A. The self-rotation function indicates the presence of four and eight molecules in the crystallographic asymmetric unit of the form I and form II crystals, respectively. Attempts to solve these structures by molecular-replacement methods have not been successful and a heavy-atom derivative search has been initiated.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherBlackwell Munksgaard
dc.publisher.placeCopenhagen, Denmark
dc.relation.ispartofpagefrom1879
dc.relation.ispartofpageto1881
dc.relation.ispartofissue10
dc.relation.ispartofjournalActa crystallographica. Section D, Biological crystallography
dc.relation.ispartofvolume58
dc.subject.fieldofresearchStructural Biology (incl. Macromolecular Modelling)
dc.subject.fieldofresearchPhysical Sciences
dc.subject.fieldofresearchChemical Sciences
dc.subject.fieldofresearchBiological Sciences
dc.subject.fieldofresearchcode060112
dc.subject.fieldofresearchcode02
dc.subject.fieldofresearchcode03
dc.subject.fieldofresearchcode06
dc.titleCrystallization and preliminary X-ray analysis of bucain, a novel toxin from the Malayan krait Bungarus candidus.
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codec1x
gro.facultyGriffith Health Faculty
gro.date.issued2002
gro.hasfulltextNo Full Text
gro.griffith.authorNirthanan, Niru


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