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  • HLA-A*0201 restricted CD8+ T-lymphocyte responses to malaria: identification of new Plasmodium falciparum epitopes by IFN-γ ELISPOT

    Author(s)
    Gonzalez, JM
    Peter, K
    Esposito, F
    Nebie, I
    Tiercy, JM
    Bonelo, A
    Arevalo-Herrera, M
    Valmori, D
    Romero, P
    Herrera, S
    Corradin, G
    Lopez, JA
    Griffith University Author(s)
    Lopez Ramirez, Alejandro
    Year published
    2000
    Metadata
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    Abstract
    The role of antigen specific CD8 T-lymphocytes in mediating protection against sporozoite-induced malaria has been well established in murine models. In humans, indirect evidence has accumulated suggesting a similar protective role for antigen-specific CD8 T-lymphocytes. Nevertheless, the low frequency of circulating specific cells together with the lack of sensitive methods to quantify them has hampered the direct assessment of their function. Using a combination of short-term cell culture and IFN-g ELISPOT, we studied CD8 T-lymphocyte responses to a panel of HLA-A*0201 binding peptides. In addition to confirming the ...
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    The role of antigen specific CD8 T-lymphocytes in mediating protection against sporozoite-induced malaria has been well established in murine models. In humans, indirect evidence has accumulated suggesting a similar protective role for antigen-specific CD8 T-lymphocytes. Nevertheless, the low frequency of circulating specific cells together with the lack of sensitive methods to quantify them has hampered the direct assessment of their function. Using a combination of short-term cell culture and IFN-g ELISPOT, we studied CD8 T-lymphocyte responses to a panel of HLA-A*0201 binding peptides. In addition to confirming the response to already described epitopes, we also identified five new CD8 T-lymphocyte epitopes. These epitopes are presented in pre-erythrocytic stages gene products of Plasmodium falciparum 7G8 strain and correspond to the following protein segments: circumsporozoite (CS) 64-72, 104-113, 299-308 and 403-411; liver stage antigen (LSA-1) repeat region; sporozoite surface protein 2 or thrombospondin related anonymous protein (SSP2/TRAP) 78±88 and 504±513. Four of these peptides are conserved mongst all published sequences of P. falciparum strains. We conclude that the modified IFN-g ELISPOT assay is a sensitive technique to monitor antigen-speci®c CD8 T-lymphocyte responses in human malaria which may help in the improvement and assessment of the efficacy of malaria subunit vaccines.
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    Journal Title
    Parasite Immunology
    Volume
    22
    Issue
    10
    DOI
    https://doi.org/10.1046/j.1365-3024.2000.00331.x
    Subject
    Cellular Immunology
    Microbiology
    Veterinary Sciences
    Medical Microbiology
    Publication URI
    http://hdl.handle.net/10072/55210
    Collection
    • Journal articles

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